| 货号 | AF5849-SP |
| 别名 | IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform | 全称 | Interleukin 21 |
| 反应种属 | Canine |
| 应用 | Neutralization |
| 目标/特异性 | Detects canine IL-21 in direct ELISAs. In direct ELISAs, approximately 35% cross-reactivity with recombinant human IL-21 is observed, and less than 3% cross-reactivity with recombinant mouse IL-21 is observed. |
| 使用方法 | Neutralization: Measured by its ability to neutralize IL‑21-induced proliferation in the N1186 human T cell line. Parrish-Novak, J. et al. (2000) Nature 408:57. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Canine IL‑21. |
| 来源 | Polyclonal Sheep IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant canine IL-21 His18-Ser146 Accession # NP_001003347 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Canine |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Interleukin-21 (IL-21) is a 14 kDa (predicted) four-helix-bundle cytokine and member of the IL-15/IL-21 family. It is made by activated CD4+ T cells, activated NKT cells, T helper (Th) cells, and Th17 cells (1‑5). Canine IL-21 is synthesized as a 146 amino acid (aa) precursor that contains a 17 aa signal sequence and a 129 aa mature chain. Mature canine IL-21 is 86%, 73%, 66%, and 64% aa identical to mature bovine, human, mouse and rat IL-21, respectively. IL-21 binds to a heterodimeric receptor, which is formed by the common gamma -chain subunit (CD134), shared with IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15 receptors, and its own receptor, IL‑21 R, a member of the class I cytokine receptor family (1‑2). IL-21 R is expressed on a variety of immune cells, including B cells, T cells, dendritic cells, and NK cells, as well as non-immune cells, such as fibroblasts, epithelial cells, and endothelial cells (1‑2, 5). Binding of IL-21 to its receptor leads to the activation of members of the JAK-family protein tyrosine kinases, JAK1 and JAK3, and signal transducer and activator of transcription (Stat) molecules (1). IL-21 regulates activation, proliferation, and survival of both CD4+ T cells and B cells, the functional activity of CD8+ T cells and NK cells, and limits the differentiation of inducible regulatory T cells and counteracts their suppressive properties on effector T cells (1, 5‑6). IL-21 also negatively regulates the maturation and function of dendritic cells (1, 5‑6). IL‑21 also plays a role in many inflammatory and autoimmune diseases, such as Crohn’s disease, Helicobacter pylori‑related gastritis, celiac disease, type I diabetes mellitus, psoriasis, rheumatoid arthritis, and systemic lupus erythematosus (1). IL‑21 is also involved in controlling chronic viral infections (7). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by IL‑21 and Neutralization by Canine IL‑21 Antibody. Recombinant Canine IL‑21 (Catalog # 5849-ML) induces proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑21 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Canine IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5849). The ND50 is typically 2-10 µg/mL. |
