Cotton Rat IL-2 Affinity Purified Polyclonal PAb (25 UG)
货号：
AF579-SP 基本售价：
1378.1 元 规格：
-
产品信息
概述| 货号 | AF579-SP |
| 别名 | aldesleukin; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF |
全称 | Interleukin 2 | | 应用 | Western Blot(0.1 µg/mL)
|
| 目标/特异性 | Detect cotton rat IL‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat IL-2 and recombinant mouse IL-2 is observed and less than 5% cross-reactivity with recombinant human IL-2 and recombinant porcine IL-2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL
Neutralization: Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A. J. H. and C. B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 1.5 ng/mL Recombinant Cotton Rat IL‑2 (Cys146Tyr).
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| 来源 | Polyclonal Goat IgG |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant cotton rat IL‑2 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Cotton Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | IL-2 (Interleukin 2) is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex. Gamma c is also a signaling subunit in the receptors for IL-4, -7, -9, -15, and -21. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - BIObrief: Common gamma-Chain Family Cytokines Play a Pivotal Role in Regulating Immune System Functions
- Cancer Biomarkers
- Common gamma Chain Receptor Family
- Common gamma-Chain Family Cytokines Play a Pivotal Role in Regulating Immune System Functions
- Conventional/Classical Dendritic Cells
- Cytokines and Receptors in Allergy and Asthma
- Cytokines and Receptors in Tfh Cell Differentiation and Function
- gamma delta T Cells
- Group 1 ILCs
- ICC / IHC Images: IL-2
- Infectious Diseases
- Inflammatory Mediators
- Interactive Pathway: IL-2 Signaling and its Primary Biological Effects in Different Immune Cell Types
- Interactive Pathway: IL-2 Signaling Pathways
- Isolation, Culture, and Identification of Natural Killer Cells
- Mini-review: Cytolytic Granule-mediated Apoptosis
- Multiple Sclerosis and Myelin Related Molecules
- NCR+ILC3
- Neuroinflammation
- Preeclampsia
- Regulatory T Cells (Tregs)
- T Cell Cytokine Signaling
- Th1 Cells
- Th2 Cells
- Th9 Cells
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参考图片Cell Proliferation Induced by IL‑2 and Neutralization by Cotton Rat IL‑2 Antibody. Recombinant Cotton Rat IL‑2 (Cys146Tyr) (Catalog # 579-R2) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL‑2 (Cys146Tyr) (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF579). The ND 50 is typically 0.1-0.4 µg/mL. |
