| 货号 | AF552-SP |
| 别名 | Hematopoietin-1; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha | 全称 | Interleukin 1 alpha |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects cotton rat IL‑1 alpha /IL‑1F1 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant rat IL-1 alpha is observed, 15% cross-reactivity with recombinant mouse IL-1 alpha is observed, and less than 1% cross-reactivity with recombinant human IL-1 alpha and recombinant porcine IL-1 alpha is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.006-0.036 µg/mL in the presence of 50 pg/mL Recombinant Cotton Rat IL‑1 alpha /IL‑1F1 and 1.25 µg/mL concanavalin A. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant cotton rat IL‑1 alpha /IL‑1F1 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Cotton Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Cotton Rat IL‑1 alpha /IL‑1F1 Antibody. Recombinant Cotton Rat IL‑1 alpha /IL‑1F1 (Catalog # 552-R1) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL‑1 alpha /IL‑1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF552). The ND50 is typically 0.006-0.036 µg/mL in the presence of concanavalin A (1.25 µg/mL). |
