| 货号 | AF535-SP |
| 别名 | 3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1 | 全称 | Interleukin 8 |
| 反应种属 | Porcine |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human CXCL8/IL‑8 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human IL-8 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CXCL8/IL‑8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1.5-7.5 µg/mL in the presence of 50 ng/mL Recombinant Porcine CXCL8/IL‑8. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Exposure to hog barn dust alters airway epithelial ciliary beating. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant porcine CXCL8/IL‑8 Ala26-Gln104 Accession # CAA43461 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Porcine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interleukin 8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc. IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro-inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. Recently, IL-8 was reported to be angiogenic both in vivoandin vitro. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Porcine CXCL8/IL‑8 Antibody. Recombinant Porcine CXCL8/IL‑8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine CXCL8/IL‑8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine CXCL8/IL‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF535). The ND50 is typically 1.5-7.5 µg/mL. |
