货号 | AF3890-SP |
别名 | CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1 |
反应种属 | Porcine |
应用 | Western Blot(0.1 µg/mL) |
目标/特异性 | Detects porcine CD34 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross‑reactivity with recombinant rat CD34 is observed, and less than 5% cross-reactivity with recombinant mouse CD34 and recombinant human CD34 is observed. |
使用方法 | Western Blot: 0.1 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine) |
纯化方式 | Antigen Affinity-purified |
免疫原 | Mouse myeloma cell line NS0-derived recombinant porcine CD34 Ala20-Thr294 (Thr32Ala, Ala52Thr, Pro71Ser, Val126Ala, His278Asn) Accession # NP_999251 |
生物活性 | Porcine |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | CD34 is a 110 kDa type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucin. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Porcine CD34 is 389 amino acids (aa) in length and is predicted to have a 272 aa extracellular domain (ECD) and a 73 aa cytoplasmic tail. An alternate 307 aa splice form with a 4 aa substitution for the C-terminal 61 amino acids also exists. Over aa 20-294, porcine CD34 shares 51%, 57% and 62% aa sequence identity with mouse, human, and canine CD34, respectively. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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