Porcine IL-5 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Porcine IL-5 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3137-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3137-SP
别名	BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I
全称	Interleukin 5
反应种属	Porcine
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects porcine IL-5 in direct ELISAs and Western blots. In direct ELISAs, 50% cross‑reactivity with recombinant canine IL‑5 and recombinant feline IL-5 is observed and 30% cross-reactivity with recombinant human IL-5, recombinant mouse IL-5, and recombinant rat IL-5 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 2-8 µg/mL in the presence of 25 ng/mL Recombinant Porcine IL‑5.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant porcine IL-5 Ile20-Ser134 Accession # Q9MYM5
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Porcine
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.
运输条件	Blue Ice
存放说明	4℃
参考文献	Common beta Chain Receptor Family Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Tfh Cell Differentiation and Function gamma delta T Cells Granulocyte Markers Group 2 ILCs ICC / IHC Images: IL-5 Inflammatory Mediators Mini-review: Interleukin 5 T Cell Cytokine Signaling Th2 Cells

参考图片

Cell Proliferation Induced by IL‑5 and Neutralization by Porcine IL‑5 Antibody. Recombinant Porcine IL‑5 (Catalog # 3137-PL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑5 (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3137). The ND₅₀ is typically 2‑8 µg/mL.