Canine IL-18/IL-1F4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Canine IL-18/IL-1F4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF2924-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2924-SP
别名	Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18
全称	Interleukin 18
反应种属	Canine
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects canine IL‑18/IL‑1F4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant porcine IL-18 is observed, 20% cross-reactivity with recombinant rhesus macaque IL-18 is observed, 15% cross-reactivity with recombinant human IL-18 is observed, and 5% cross-reactivity with recombinant mouse IL-18 and recombinant rat IL-18 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant canine IL‑18/IL‑1F4 Tyr37-Ser193 Accession # Q9XSR0
生物活性	Canine
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-18, also known as interferon-gamma-inducing factor (IGIF) and IL-18, is an 18 kDa protein belonging to the IL-1 superfamily. It is synthesized as a 24 kDa pro-IL-18 and is processed by caspase 1 to form the active protein. IL-18 is produced by multiple cell types including monocyte/macrophages, dendritic cells, osteoblasts and keratinocytes. Mature IL-18 synergizes with IL-12 to induce differentiation of naïve Th cells into Th1 cells. It also plays multiple roles in other immune cell types including macrophages, NK cells and B cells. Canine and human mature IL-18 share 73% amino acid sequence identity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cytokines and Receptors in Angiogenesis Group 1 ILCs IL-1 Family Inflammasomes: Intracellular Regulators of Pathogen Interactive Pathway: IL-1 Family Signaling Pathways Isolation, Culture, and Identification of Natural Killer Cells Macrophage Activation Markers Mini-review: Interleukin 18 (IL-18) Multiple Sclerosis and Myelin Related Molecules Neuroinflammation NOD-like Receptors and the Inflammasome T Cell Cytokine Signaling Th1 Cells The Nlrp3 Inflammasome & IL-18 Regulate Intestinal Homeostasis

参考图片

IL‑18/IL‑1F4 in Canine PBMCs.
IL‑18/IL‑1F4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine
IL‑18/IL‑1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2924) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretions. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.