| 货号 | AF2598-SP |
| 别名 | CD8; CD8A |
| 反应种属 | Feline |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects feline CD8 alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse CD8 alpha is observed, approximately 5% cross-reactivity with recombinant canine CD8 alpha is observed, and less than 1% cross-reactivity with recombinant human CD8 alpha is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 925 (Human); 12525 (Mouse); 24930 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant feline CD8 alpha Ala22-Tyr188 Accession # P41688 |
| 生物活性 | Feline |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CD8 is a dimeric complex made up of two Ig superfamily members. The CD8 alpha chain is a 34 kDa type I transmembrane glycoprotein that is disulfide-linked, either to itself forming CD8 alpha alpha, or to an unrelated 30-35 kDa CD8 beta chain, forming CD8 alpha beta. CD8 alpha contains one V-type Ig-like domain in its extracellular region that binds to class I MHC molecules. CD8 alpha beta is a TCR coreceptor, while CD8 alpha alpha promotes T cell survival and differentation. Feline CD8 alpha extracellular region shares 58%, 47%, 51%, and 69% amino acid sequence identity with human, mouse, porcine, and canine CD8 alpha extracellular regions, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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CD8 alpha in Feline PBMCs. CD8 alpha was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline CD8 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2598) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
