| 货号 | AF2467-SP |
| 别名 | FL; Flt3 ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine | 全称 | fms-like Tyrosine Kinase 3 Ligand |
| 反应种属 | Feline |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects feline Flt‑3 Ligand in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross‑reactivity with recombinant human Flt-3 Ligand and recombinant mouse Flt-3 Ligand is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize Flt‑3 Ligand-induced proliferation in BaFlt3 mouse pro‑B cell line transfected with mouse Flt‑3. Hannum, C. et al. (1994) Nature 368:643. The Neutralization Dose (ND50) is typically 0.1‑0.4 µg/mL in the presence of 2 ng/mL Recombinant Feline Flt‑3 Ligand. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2323 (Human); 14256 (Mouse); 493796 (Feline) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant feline Flt-3 Ligand Ser27-Pro185 Accession # NP_001009842 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Feline |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Flt-3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1‑3). Mature feline Flt-3 Ligand consists of a 156 amino acid (aa) extracellular region with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and an 88 aa cytoplasmic tail (4, 5). Within the ECD, feline Flt-3 Ligand shares 92%, 81%, 70%, and 63% aa sequence identity with canine, human, mouse, and rat Flt-3 Ligand, respectively. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 6). Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates (6). Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain (6, 7). Multiple splice forms of canine, human, and mouse Flt-3 Ligand have been described and may also exist in feline (4, 6‑10). Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3, 5, 7, 10). Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 11). It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (7, 8, 10). It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation (1, 12). Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation (13). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by Flt‑3 Ligand and Neutralization by Feline Flt‑3 Ligand Antibody. Recombinant Feline Flt‑3 Ligand (Catalog # 2467-FL) stimulates proliferation in BaFlt3 mouse pro‑B cell line transfected with mouse Flt‑3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline Flt‑3 Ligand (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-FelineFlt-3 Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2467). The ND50 is typically 0.1-0.4 µg/mL. | |
Flt‑3 Ligand in Feline PBMCs. Flt‑3 Ligand was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-FelineFlt‑3 Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2467) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
