| 货号 | AF2465-SP |
| 别名 | aldesleukin; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF | 全称 | Interleukin 2 |
| 反应种属 | Bovine |
| 应用 | Western Blot,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard |
| 目标/特异性 | Detects bovine IL-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human IL-2, recombinant mouse IL-2, recombinant rat IL-2, recombinant feline IL-2, recombinant canine IL-2, recombinant equine IL-2, recombinant cotton rat IL-2, and recombinant porcine IL-2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant bovine IL‑2 Ala21-Thr155 Accession # P05016 |
| 生物活性 | Bovine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Bovine Interleukin-2 (IL-2) is a 15 kDa, alpha -helical, single chain, potentially glycosylated polypeptide that has potent stimulatory activity for antigen-activated T cells (1‑5). The molecule is synthesized as a 155 amino acid (aa) precursor that contains a 20 aa signal peptide plus a 135 aa mature segment that is possibly O‑glycosylated (4, 5). The mature region has multiple alpha -helices and one intrachain disulfide bond. Mature bovine IL-2 is 64%, 60%, 49%, 50%, 72%, 63% and 67% to mature human, canine, mouse, rat, porcine, equine, and feline IL-2, respectively. Mammalian cells known to express IL-2 include CD4+ and CD8+ T cells, visceral smooth muscle cells, eosinophils, gamma δ T cells, B cells and dendritic cells. The receptor for IL-2 is complex and consists of three distinct subunits in varying combinations (6, 7). Two of these are ligand-binding and are termed IL-2 R alpha and IL-2 R beta. IL-2 R alpha is 55 kDa and binds IL-2 with low affinity. IL-2 R beta is 75 kDa and binds IL-2 with intermediate affinity. Signal transduction is performed by both IL-2 R beta and a 64 kDa common gamma chain ( gamma c). This signal transducing common gamma chain does not bind IL-2, but does heterodimerize with IL-2 R beta to form a functional IL-2 receptor. The complex heterotrimeric alpha -beta -gamma c receptor may arise from IL-2 binding to preformed R alpha -R beta complexes (8). Functionally, IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells into active G1, inducing IL-2 and IL-2 R alpha synthesis and cell proliferation (7). It also promotes Fas-induced death of naïve CD4+ T cells, while having minimal effect on activated CD4+ memory lymphocytes. Finally, IL-2 seems to play a central role in the expansion and maintenance of CD4+CD25+ regulatory T cells. Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (9, 10). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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IL‑2 in Bovine PBMCs. IL‑2 was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Bovine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2465) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
