| 货号 | AF2118-SP |
| 别名 | CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35 | 全称 | Interleukin 12 |
| 反应种属 | Canine |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects canine IL-12 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human IL‑12 p35/p40 and recombinant feline IL-12 p35/p40 is observed, and less than 10% cross-reactivity with recombinant rat IL-12 p35/p40 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3592 (Human); 16159 (Mouse); 84405 (Rat); 403977 (Canine); 493741 (Feline) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant canine IL-12 p40: Ile23-Ser329; p35: Arg26-Ser222 (Asp63Glu, Tyr64Leu, Ile65Tyr, Pro66Ser) Accession # p40: Q28268; p35: Q28267 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Canine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interleukin 12 (IL-12) is the founding member of the IL-12 family of heterodimeric cytokines, which have important immunological functions. IL-12 is composed of two disulfide-linked subunits of 35 kDa and 40 kDa, respectively. The 35 kDa subunit (p35) is an alpha -helical protein homologous to IL-6 and G-CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also utilized in IL-23. Mature canine p35 is a 197 amino acids (aa) protein that is secreted as a heterodimer linked to p40. It contains two potential N-linked glycosylation sites and shares 91%, 86%, 85%, 56%, and 55% aa sequence identity with feline, human, porcine, rat and mouse p35, respectively. Mature p40 contains 307 aa and can exist in multiple forms, including monomer, homodimer, heterodimer linked to p19 (forming IL-23), and heterodimer linked to p35 (forming IL-12). It is upregulated by substances such as LPS and CpG that activate antigen-presenting cells. Mature canine p40 shows 94%, 85%, 84%, 65%, and 65% aa sequence identity to feline, human, porcine, rat and mouse p40, respectively. Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. The activities of IL-12 are mediated by the receptor complex composed of two type I transmembrane proteins: a 100 kDa ligand-binding subunit (IL-12 R beta 1) and a 130 kDa signal transducing subunit (IL-12 R beta 2). IL-12 facilitates hematopoietic stem cell proliferation, induces NK cell proliferation, promotes IgG production (in mice), and potentiates the expansion and late activation of Th1 CD4+ T cells (1‑5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
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