| 货号 | AF1964-SP |
| 别名 | BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I | 全称 | Interleukin 5 |
| 反应种属 | Canine |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects canine canine IL‑5 in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant bovine IL-5, recombinant equine IL-5, and recombinant feline IL-5 is observed and less than 5% cross-reactivity with recombinant rat IL-5, recombinant mouse IL-5, and recombinant human IL-5 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.75-3.0 µg/mL in the presence of 30 ng/mL Recombinant Canine IL‑5. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant canine IL-5 Phe20-Ser134 Accession # Q95J76 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Canine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interleukin-5 (IL-5) is a 40‑45 kDa secreted disulfide-linked homodimeric glycoprotein that plays an important role in the differentiation, growth, and function of eosinophils. It also primes basophils for histamine and leukotriene release. In mice, IL-5 also induces the proliferation, differentiation, and immunoglobulin production of B cells especially B-1 cells that constitutively express IL-5 receptor alpha. IL-5 is primarily produced by CD4+ Th2 cells. Other cell types, including mast cells, visceral smooth muscle cells, bronchial epithelium, CD16+ NK cells, eosinophils and gamma δ T cells, can also produce IL-5. Canine IL-5 is synthesized as a 134 amino acid (aa) precursor that contains a 21 aa signal sequence and a 113 aa mature segment. Mature canine IL-5 shares 62%, 66%, 85%, 84%, 58%, and 56% aa sequence identity with mature human, guinea pig, porcine, feline, mouse, and rat IL-5, respectively. The receptor for IL-5 consists of a 60 kDa ligand-binding subunit (IL-5 R alpha ) and a 120 kDa signal-transducing subunit ( beta c) (1‑7). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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IL‑5 in Canine PBMCs. IL‑5 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Goat Anti-Canine IL‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1964) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. | |
Cell Proliferation Induced by IL‑5 and Neutralization by Canine IL‑5 Antibody. Recombinant Canine IL‑5 (Catalog # 1964-CL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑5 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL‑5 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF1964). The ND50 is typically 0.75-3.0 µg/mL. |
