| 货号 | AF1605-SP |
| 别名 | CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF | 全称 | Interleukin 10 |
| 反应种属 | Equine |
| 应用 | Western Blot,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization |
| 目标/特异性 | Detects equine IL‑10 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant canine IL-10 and recombinant porcine IL-10 is observed and less than 0.4% cross-reactivity with recombinant human IL-10, recombinant mouse IL-10, recombinant rat IL-10, and recombinant feline IL-10 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 10-25 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. The Neutralization Dose (ND50) is typically 0.2-0.6 µg/mL in the presence of 20 ng/mL Recombinant Equine IL‑10. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant equine IL-10 Ser19-Asn178 Accession # Q28374 |
| 内毒素水平 | <0.15 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Equine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of mouse T helper 2 (Th2) cells that inhibited the cytokine production by Th1 cells. It is a pleiotropic cytokine that regulates the immune and inflammatory responses of hematopoietic cells (1, 2). IL-10 has immunosuppressive activities and has been shown to inhibit the effector functions of monocyte/macrophage and CD4+T cells. Conversely, IL-10 has immunostimulatory activities and can induce the proliferation and cytotoxic activity of CD8+T cells and NK cells. IL-10 also regulates the growth and differentiation of B cells, mast cells, dendritic cells and neutrophils (1). The biological activities of IL-10 is mediated by the heteromeric IL-10 receptor complex, which is composed of the ligand-binding IL-10R alpha and the accessory IL-10R beta subunits. Both subunits belong to the class II cytokine receptor family. IL-10R beta is also utilized as a subunit in the heterodimer receptor complex for IL-22, IL-28 and IL-29. Besides IL-10, five novel cytokines (IL-19, -20, -22, -24, and -26) that share structural and limited sequence homology with IL-10 have been identified. These proteins constitute the IL-10 cytokine family (3). Equine IL-10 cDNA encodes a 178 amino acid residue (aa) precursor protein with an 18 aa signal peptide and 160 aa mature protein that contains two potential N-linked glycosylation sites. Analogous to human IL-10, equine IL-10 likely exists as nondisulfide-linked homodimers. Equine IL-10 shares 71% and 78% aa sequence homology with mouse and human IL-10, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by IL‑10 and Neutralization by Equine IL‑10 Antibody. Recombinant Equine IL‑10 (Catalog # 1605-IL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Equine IL‑10 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1605). The ND50 is typically 0.2-0.6 µg/mL. | |
IL‑10 in Equine PBMCs. IL‑10 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Goat Anti-Equine IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1605) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
