| 货号 | AF128-SP |
| 别名 | netrin 1; netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of |
| 反应种属 | Chicken |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects chicken Netrin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant chicken Netrin‑2 and recombinant mouse Netrin-1 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9423 (Human); 18208 (Mouse); 396389 (Chicken) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Netrin-1 overexpression protects kidney from ischemia reperfusion injury by suppressing apoptosis. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant chicken Netrin-1 (R&D Systems, Catalog # 128-N1) Gly26-Ala606 Accession # Q90922 |
| 生物活性 | Chicken |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Chicken Netrin-1 is the prototypical member of an ever-expanding, laminin-related family of axon-guidance molecules collectively referrred to as netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 606 amino acid (aa) protein precursor that has structural similarity to the N-terminus of the B2 or gamma ‑chain of laminin. It contains one 250 aa type VI globular domain, three type V (~55 aa) cysteine/glycine rich EGF repeats, and one unique 140 aa “C” domain that binds heparin. Chick Netrin-1 shares 78% aa identity with chicken Netrin-2 and 86% aa identity with mouse and human Netrin-1. Although only two chick netrins are known, the number of known mammalian netrins is increasing. Human and mouse Netrin-3/NTN-2L, and a mouse Netrin-4, that shares homology with the B1 or beta ‑chain of laminin, have been reported. Cells reported to express Netrin-1 in the embryo include cells of the spinal cord floor plate and somite, cells of the ganglionic eminence, and cells of the floor plate of the met- and caudal mesencephalon. In the adult, neurons of the thalamus, neocortex, and hippocampus, plus Schwann cells, osteoclasts and osteoblasts all reportedly produce Netrin-1. The DCC (deleted in colorectal cancer) gene product as well as the UNC5 family of receptors and the adenosine A2b receptor have been proposed to be functional receptors for Netrin-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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