| 货号 | AF1082-SP |
| 别名 | IFNalpha; IFN-alpha | 全称 | Interferon alpha |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects cotton rat IFN-alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human IFN-beta and recombinant cotton rat IFN-gamma is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IFN‑ alpha inhibition of EMCV-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 0.015-0.065 µg/mL in the presence of 0.2 ng/mL Recombinant Cotton Rat IFN‑ alpha (Cys109Tyr). |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3440 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Synergistic induction of interferon alpha through TLR-3 and TLR-9 agonists identifies CD21 as interferon alpha receptor for the B cell response. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant cotton rat IFN-alpha Cys24-Lys190 (Cys109Tyr) Accession # AAL18816 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Cotton Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interferon-alpha, also known as leukocyte interferon, comprises a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R1) and a 125 kDa subunit (IFN-alpha R2) that is involved both in ligand-binding and signal transduction. IFN-alpha is expressed primarily by leukocytes upon activation by viruses, bacteria, cytokines and growth factors. IFN-alpha has been shown to have anti-viral and immunomodulatory activities on target cells. It can also inhibit cell proliferation as well as induce apoptosis (1, 2). Cotton rat IFN-alpha 1 cDNA encodes a 189 amino acid (aa) residue precursor protein with a putative 23 aa signal sequence and a 166 aa mature protein. The protein contains five cysteines, four of which are involved in two intrachain disulfide bonds. Cotton rat IFN-alpha 1 shares 52%, 67%, 68% and 72% aa sequence identity to human, rat, hamster and mouse IFN-alpha 1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
IFN‑ alpha Inhibition of EMCV-induced Cytopathy and Neutralization by Cotton Rat IFN‑ alpha Antibody. Recombinant Cotton Rat IFN‑ alpha (Cys109Tyr) (Catalog # 1082‑CR) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Cotton Rat IFN‑ alpha (Cys109Tyr) (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1082). The ND50 is typically 0.015‑0.065 µg/mL. |
