| 货号 | AF1061-SP |
| 别名 | C-C motif chemokine 3; MIP1-(a); AI323804; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP-1 alpha; Mip1a; MIP-1alpha; MIP1-alpha; Scya3 |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects cotton rat CCL3/MIP‑1 alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse MIP-1 alpha is observed, 15% cross-reactivity with recombinant human (rh) MIP-1 alpha is observed, less than 10% cross-reactivity with rhMIP‑1 beta, rh6Ckine, and rhHCC-4 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 10 ng/mL Recombinant Cotton Rat CCL3/MIP‑1 alpha. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant cotton rat CCL3/MIP-1 alpha Ala24-Ala92 Accession # AAL26704 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Cotton Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | MIP-1 alpha is a beta family (CC) chemokine and has been designated CCL3. MIP-1 alpha and MIP-1 beta, two closely related but distinct proteins, were originally purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Cotton rat MIP-1 alpha cDNA encodes a 92 amino acid (aa) residue precursor protein with a 23 aa putative signal peptide. Mature cotton rat MIP-1 alpha shares approximately 70% amino acid identity with human MIP-1 alpha. MIP-1 alpha is expressed in a wide variety of cells, including lymphocytes, fibroblasts, and epithelial cells, as well as monocytes/macrophages. MIP-1 alpha has been shown to play an important role in the recruitment of mononuclear cells. Additionally, MIP-1 alpha has been reported to have chemoattractant and adhesive effects on lymphocytes, preferentially promoting the chemotaxis of Th1 cells. MIP-1 alpha has also been shown to attract B cells, eosinophils, and dendritic cells. In addition, MIP-1 alpha augments cytolytic activity of NK cells (1). MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. It has been demonstrated that MIP-1 alpha can bind the chemokine receptors CCR1 and CCR5 (2). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Cotton Rat CCL3/MIP‑1 alpha Antibody. Recombinant Cotton Rat CCL3/MIP‑1 alpha (Catalog # 1061‑RM) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL3/MIP‑1 alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat CCL3/MIP‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1061). The ND50 is typically 0.1-0.4 µg/mL. |
