| 货号 | AF1024-SP |
| 别名 | ACT2; ACT-2; AT744.1; C-C motif chemokine 4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2 |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects cotton rat CCL4/MIP‑1 beta in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human MIP-1 beta is observed and less than 2% cross-reactivity with recombinant mouse MIP-1 beta is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CCL4/MIP‑1 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR5. The Neutralization Dose (ND50) is typically 0.15-0.6 µg/mL in the presence of 10 ng/mL Recombinant Cotton Rat CCL4/MIP‑1 beta. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant cotton rat CCL4/MIP-1 beta Ala24-Asn92 Accession # AAL16933 |
| 内毒素水平 | <0.01 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Cotton Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CCL4, also known as macrophage inflammatory protein 1 beta (MIP-1 beta ), is a 12 kDa beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells (1, 2). CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium (3 - 8). A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment (6). CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha (9). The first two N-terminal amino acids (aa) can be cleaved from human CCL4 by CD26/DPPIV (10, 11). Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2 (10). In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor (2). Both forms of CCL4 block HIV-1 infection of T cells by inducing the downregulation of CCR5 (10). Mature cotton rat CCL4 shares 75% - 83% aa sequence identity with human, mouse, and rat CCL4. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by CCL4/MIP‑1 beta and Neutralization by Cotton Rat CCL4/ MIP‑1 beta Antibody. Recombinant Cotton Rat CCL4/MIP‑1 beta (Catalog # 1024-MB) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL4/MIP‑1 beta (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat CCL4/ MIP‑1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1024). The ND50 is typically 0.15-0.6 µg/mL. |
