Cotton Rat IL-1 beta/IL-1F2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Cotton Rat IL-1 beta/IL-1F2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1009-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1009-SP
别名	catabolin; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
全称	Interleukin 1 beta
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects cotton rat IL‑1 beta /IL‑1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human IL-1 beta and recombinant mouse IL-1 beta is observed, and 10% cross‑reactivity with recombinant rat IL-1 beta and recombinant porcine IL-1 beta is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 0.4-1.2 µg/mL in the presence of 2 ng/mL Recombinant Cotton Rat IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant cotton rat IL‑1 beta
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Cotton Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers Chronic IL-1 beta-induced Neuroinflammation Is it Really that Bad? Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis ICC / IHC Images: IL-1 beta/IL-1F2 IL-1 Family Infectious Diseases Inflammasomes: Intracellular Regulators of Pathogen Inflammatory Mediators Interactive Pathway: IL-1 Family Signaling Pathways Isolation, Culture, and Identification of Natural Killer Cells LTi Cells Macrophage Activation Markers Mini-review: Interleukin 1 Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) NCR-ILC3 Neuroinflammation NOD-like Receptors and the Inflammasome Platelets Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th17 Cells Th9 Cells

参考图片

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Cotton Rat IL‑1 beta /
IL‑1F2 Antibody.
Recombinant Cotton Rat IL‑1 beta /IL‑1F2 (Catalog # 1009‑CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose‑dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL‑1 beta /IL‑1F2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL‑1 beta /
IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1009). The ND₅₀ is typically 0.4-1.2 µg/mL in the presence of concanavalin A (1.25 µg/mL).