| 货号 | AF982-SP |
| 别名 | CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects mouse CD14 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human CD14 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Sialic Acid within the Glycosylphosphatidylinositol Anchor Targets the Cellular Prion Protein to Synapses | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse CD14 Ala18-Pro345 Accession # Q4FJP7 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The mouse CD14 cDNA encodes a 366 amino acid (aa) residue precursor protein with a 15 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Mouse CD14 has five potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of mouse CD14 is approximately 65% and 82% identical to the human and rat proteins, respectively. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Mouse CD14 by Western Blot. Western blot shows lysate of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse CD14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF982) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD14 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
