Mouse MMP-9 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse MMP-9 Affinity Purified Polyclonal Ab (25 UG)

货号： AF909-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF909-SP
别名	92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP-9; type V collagenase
全称	Matrix Metalloproteinase 9
反应种属	Mouse
应用	Western Blot(.25 µg/mL) Simple Western(5 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL)
目标/特异性	Detects mouse MMP-9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) MMP-9 is observed and less than 2% cross-reactivity with rhMMP-1, -2, -3, -7, -8, -10, -12, and -13 is observed.
使用方法	Western Blot: .25 µg/mL Simple Western: 5 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4318 (Human); 17395 (Mouse); 81687 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Immunodominant fragments of myelin basic protein initiate T cell-dependent pain. J Neuroinflammation, 2102;9(0):119. Species: Mouse Sample Type: Whole Tissue Application: IHC Thymocyte development in the absence of matrix metalloproteinase-9/gelatinase B Sci Rep, 2016;6(0):29852. Species: Mouse Sample Type: Whole Cells Application: Flow Matrix Metalloproteinase-2 (MMP-2) Gene Deletion Enhances MMP-9 Activity, Impairs PARP-1 Degradation, and Exacerbates Hepatic Ischemia and Reperfusion Injury in Mice. Authors: Kato H, Duarte S, Liu D, Busuttil R, Coito A PLoS ONE, 2015;10(9):e0137642. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified Cytomegalovirus initiates infection selectively from high-level beta1 integrin-expressing cells in the brain. Authors: Kawasaki H, Kosugi I, Sakao-Suzuki M, Meguro S, Arai Y, Tsutsui Y, Iwashita T Am J Pathol, 2015;185(5):1304-23. Species: Mouse Sample Type: Whole Cells Application: WB Possible dual role of decorin in abdominal aortic aneurysm. Authors: Ueda, Koshiro, Yoshimura, Koichi, Yamashita, Osamu, Harada, Takasuke, Morikage, Noriyasu, Hamano, Kimikazu PLoS ONE, 2015;10(3):e0120689. Species: Mouse Sample Type: Whole Tissue Application: IHC Hepatic ischemia and reperfusion injury in the absence of myeloid cell-derived COX-2 in mice. Authors: Duarte, Sergio, Kato, Hiroyuki, Kuriyama, Naohisa, Suko, Kathryn, Ishikawa, Tomo-O, Busuttil, Ronald W, Herschman, Harvey R, Coito, Ana J PLoS ONE, 2014;9(5):e96913. Species: Mouse Sample Type: Whole Tissue Application: IHC OCT-embedded MMP-9 deficiency shelters endothelial PECAM-1 expression and enhances regeneration of steatotic livers after ischemia and reperfusion injury. Authors: Kato H, Kuriyama N, Duarte S, Clavien P, Busuttil R, Coito A J Hepatol, 2014;60(5):1032-9. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded KLF4 regulates abdominal aortic aneurysm morphology and deletion attenuates aneurysm formation. Authors: Salmon M, Johnston W, Woo A, Pope N, Su G, Upchurch G, Owens G, Ailawadi G Circulation, 2013;128(11):S163-74. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Chemerin neutralization blocks hematopoietic stem cell osteoclastogenesis. Authors: Muruganandan S, Dranse H, Rourke J, McMullen N, Sinal C Stem Cells, 2013;31(10):2172-82. Species: Mouse Sample Type: Cell Lysates Application: WB Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice. Authors: Xue M, Dervish S, Harrison LC, Fulcher G, Jackson CJ J. Biol. Chem., 2012;287(20):16356-64. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded The MMP-9/TIMP-1 axis controls the status of differentiation and function of myelin-forming Schwann cells in nerve regeneration. Authors: Kim Y, Remacle AG, Chernov AV PLoS ONE, 2012;7(3):e33664. Species: Mouse Sample Type: Tissue Homogenates Application: WB Osteopontin-Stimulated Expression of Matrix Metalloproteinase-9 Causes Cardiomyopathy in the mdx Model of Duchenne Muscular Dystrophy. Authors: Dahiya S, Givvimani S, Bhatnagar S J. Immunol., 2011;187(5):2723-31. Species: Mouse Sample Type: Tissue Homogenates Application: WB Tissue inhibitor of matrix metalloproteinase 1 (TIMP1) controls adipogenesis in obesity in mice and in humans. Authors: Meissburger B, Stachorski L, Roder E, Rudofsky G, Wolfrum C Diabetologia, 2011;54(6):1468-79. Species: Mouse Sample Type: Tissue Homogenates Application: WB TRE17/USP6 oncogene translocated in aneurysmal bone cyst induces matrix metalloproteinase production via activation of NF-kappaB. Authors: Ye Y, Pringle LM, Lau AW Oncogene, 2010;29(25):3619-29. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Matrix metalloproteinase-9 regulates tumor cell invasion through cleavage of protease nexin-1. Authors: Xu D, McKee CM, Cao Y Cancer Res., 2010;70(17):6988-98. Species: Mouse Sample Type: cell lysaets Application: WB Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease. Authors: Schneider DJ, Lindsay JC, Zhou Y, Molina JG, Blackburn MR FASEB J., 2010;24(1):70-80. Species: Mouse Sample Type: Whole Tissue Application: IHC Fresh Tumor necrosis factor-alpha regulates distinct molecular pathways and gene networks in cultured skeletal muscle cells. Authors: Bhatnagar S, Panguluri SK, Gupta SK, Dahiya S, Lundy RF, Kumar A PLoS ONE, 2010;5(10):e13262. Species: Mouse Sample Type: Cell Lysates Application: WB Inducible nitric oxide synthase deficiency impairs matrix metalloproteinase-9 activity and disrupts leukocyte migration in hepatic ischemia/reperfusion injury. Authors: Hamada T, Duarte S, Tsuchihashi S, Busuttil RW, Coito AJ Am. J. Pathol., 2009;174(6):2265-77. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Matrix metalloproteinase-9 deficiency worsens lung injury in a model of bronchopulmonary dysplasia. Authors: Lukkarinen H, Hogmalm A, Lappalainen U, Bry K Am. J. Respir. Cell Mol. Biol., 2009;41(1):59-68. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Molecular role of Cx37 in advanced atherosclerosis: a micro-array study. Authors: Derouette JP, Wong C, Burnier L, Morel S, Sutter E, Galan K, Brisset AC, Roth I, Chadjichristos CE, Kwak BR Atherosclerosis, 2009;206(1):69-76. Species: Mouse Sample Type: Whole Tissue Application: IHC A TLR2 agonist in German cockroach frass activates MMP-9 release and is protective against allergic inflammation in mice. Authors: Page K, Ledford JR, Zhou P, Wills-Karp M J. Immunol., 2009;183(5):3400-8. Species: Mouse Sample Type: BALF Application: WB A novel method to establish microglia-free astrocyte cultures: comparison of matrix metalloproteinase expression profiles in pure cultures of astrocytes and microglia. Authors: Crocker SJ, Frausto RF, Whitton JL, Milner R Glia, 2008;56(0):1187-98. Species: Mouse Sample Type: Whole Cells Application: ICC A3 adenosine receptor signaling influences pulmonary inflammation and fibrosis. Authors: Morschl E, Molina JG, Volmer JB, Mohsenin A, Pero RS, Hong JS, Kheradmand F, Lee JJ, Blackburn MR Am. J. Respir. Cell Mol. Biol., 2008;39(6):697-705. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis. Authors: De Palma AM, Verbeken E, Van Aelst I, Van den Steen PE, Opdenakker G, Neyts J Virology, 2008;382(1):20-7. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Cyclooxygenase-2 deficiency enhances Th2 immune responses and impairs neutrophil recruitment in hepatic ischemia/reperfusion injury. Authors: Hamada T, Tsuchihashi S, Avanesyan A, Duarte S, Moore C, Busuttil RW, Coito AJ J. Immunol., 2008;180(3):1843-53. Species: Mouse Sample Type: Tissue Homogenates Application: WB Genetic deficiency of heme oxygenase-1 impairs functionality and form of an arteriovenous fistula in the mouse. Authors: Juncos JP, Tracz MJ, Croatt AJ, Grande JP, Ackerman AW, Katusic ZS, Nath KA Kidney Int., 2008;74(1):47-51. Species: Mouse Sample Type: Tissue Homogenates Application: WB Angiostatin inhibits monocyte/macrophage migration via disruption of actin cytoskeleton. Authors: Perri SR, Annabi B, Galipeau J FASEB J., 2007;21(14):3928-36. Species: Mouse Sample Type: Cell Lysates Application: WB Functional relevance of the IL-23-IL-17 axis in lungs in vivo. Authors: Ivanov S, Bozinovski S, Bossios A, Valadi H, Vlahos R, Malmhall C, Sjostrand M, Kolls JK, Anderson GP, Linden A Am. J. Respir. Cell Mol. Biol., 2007;36(4):442-51. Species: Mouse Sample Type: Whole Cells Application: ICC A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex. Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J Blood, 2007;109(5):2234-42. Species: Mouse Sample Type: Cell Lysates Application: WB Dendritic cell transmigration through brain microvessel endothelium is regulated by MIP-1alpha chemokine and matrix metalloproteinases. Authors: Zozulya AL, Reinke E, Baiu DC, Karman J, Sandor M, Fabry Z J. Immunol., 2007;178(1):520-9. Species: Mouse Sample Type: Whole Cells Application: ICC Matrix metalloproteinase-2 (MMP-2) regulates astrocyte motility in connection with the actin cytoskeleton and integrins. Authors: Ogier C, Bernard A, Chollet AM, LE Diguardher T, Hanessian S, Charton G, Khrestchatisky M, Rivera S Glia, 2006;54(4):272-84. Species: Mouse Sample Type: Whole Cells Application: ICC Expression of matrix metalloproteinases subsequent to urogenital Chlamydia muridarum infection of mice. Authors: Ramsey KH, Sigar IM, Schripsema JH, Shaba N, Cohoon KP Infect. Immun., 2005;73(10):6962-73. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Interleukin-17 as a recruitment and survival factor for airway macrophages in allergic airway inflammation. Authors: Sergejeva S, Ivanov S, Lotvall J, Linden A Am. J. Respir. Cell Mol. Biol., 2005;33(3):248-53. Species: Mouse Sample Type: Whole Cells Application: ICC Upregulation of MMP-9/TIMP-1 enzymatic system in eosinophilic meningitis caused by Angiostrongylus cantonensis. Authors: Chen KM, Lee HH, Chou HL, Liu JY, Tsai BC, Lai SC Int J Exp Pathol, 2005;86(2):81-9. Species: Mouse Sample Type: Tissue Homogenates Application: WB Ultrastructural localization of matrix metalloproteinase-9 in eosinophils from the cerebrospinal fluid of mice with eosinophilic meningitis caused by Angiostrongylus cantonensis. Authors: Tseng YK, Tu WC, Lee HH, Chen KM, Chou HL, Lai SC Ann Trop Med Parasitol, 2004;98(8):831-41. Species: Mouse Sample Type: CSF Application: Electron Microscopy
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse MMP‑9 Ala20-Pro730 Accession # P41245
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin, and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages, and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 may be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. Compared to the human MMP-9 (Catalog #911-MP), the mouse enzyme contains extra sequences in the linker region and in the hemopexin-like domain, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling APP Cleavage and Amyloid-beta Degradation Cancer Biomarkers Cell to Cell Adhesion Disassembly During EMT Collagen Modifying Enzymes Extracellular Matrix Remodeling and Cell Migration During EMT Gametes and Fertilization ICC / IHC Images: MMP-9 Inflammatory Mediators Mesenchymal Cells and EMT Metalloproteases and Regulators MMP Modulation of Cytokines MMP-12 in Smoking-Related Pulmonary Disease MMPs, TIMPs and Related Molecules New Tools: Active MMP Activity Assays OSM Induction of Chondrocyte MMP and TIMP-3 Gene Expression Preeclampsia

参考图片

Detection of Mouse MMP‑9 by Western Blot. Western blot shows lysates of mouse spleen tissue and mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMP‑9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MMP‑9 in Rat Brain. MMP‑9 was detected in perfusion fixed frozen sections of rat brain (cingulate cortex) using 1.7 µg/mL Goat Anti-Mouse MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse MMP‑9 by Simple Western^TM. Simple Western lane view shows lysates of mouse lung tissue and mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for MMP‑9 at approximately 155 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse MMP‑9 Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF909) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.