| 货号 | AF8277-SP |
| 别名 | delta-like 1 homolog (Drosophila); DLK; DLK1; DLK-1; FA1; FA1fetal antigen 1; pG2; pG2delta-like homolog (Drosophila); preadipocyte factor 1; PREF1; Pref-1; protein delta homolog 1; secredeltin; ZOG | 全称 | Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Pref‑1/DLK1/FA1 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant human Pref-1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8788 (Human); 13386 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Pref-1/DLK1/FA1 Ala24-Gln305 Accession # Q09163 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Pref-1 (Preadipocyte factor 1, Protein delta homolog 1, DLK1, FA1 and Fetal antigen 1) is a 45-60 kDa transmembrane glycoprotein that is highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary. Expression of Pref-1 is elevated in liver after birth but starts to decline around postnatal day 16. It contains 6 EGF-like domains and is involved in embryonic skeletal system development. Pref-1 inhibits preadipocyte proliferation by regulating their entry into G1/S-phase and represses preadipocyte differentiation. It is a master regulator of preadipocyte homeostasis and adipose tissue expansion. Pref-1 manipulation may, therefore, be utilized in obesity treatments. Mouse Pref-1 shares 81% aa identity with human Pref-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Pref‑1/DLK1/FA1 by Western Blot. Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Pref‑1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pref‑1/DLK1/FA1 at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Pref‑1/DLK1/FA1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry. 3T3‑L1 mouse embryonic fibroblast adipose-like cell line was stained with Goat Anti-Mouse Pref‑1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8277, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). | |
Pref‑1/DLK1/FA1 in Mouse Embryo. Pref‑1/DLK1/FA1 was detected in immersion fixed frozen sections of mouse embryonic lung using Goat Anti-Mouse Pref‑1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8277) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
