| 货号 | AF815-SP |
| 别名 | BAG family molecular chaperone regulator 1; BAG-1; Bcl-2 associating athanogene-1 protein; Bcl-2-associated athanogene 1; BCL2-associated athanogene; Bcl-2-binding protein; glucocortoid receptor-associated protein RAP46,46-KD; HAP; RAP46 | 全称 | Bcl-2-associated Anthanogene-1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(5 µg/mL) Immunoprecipitation(2 µg/106cells) |
| 目标/特异性 | Detects mouse Bag-1 in Western blots. Does not react with human Bag-1. |
| 使用方法 | Western Blot: 5 µg/mL Immunoprecipitation: 2 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 573 (Human); 12017 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Bag-1 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Bag-1 (Bcl-2-associated Athanogene 1) is a 31-kDa protein with an apparent molecular mass of 46 kDa that can bind to Bcl-2 and enhance its anti-apoptotic effect, resulting in increased protection from cell death induced by apoptotic stimuli. Bag-1 shares no significant homology with Bcl-2 or other Bcl-2 family proteins. The C-terminal region of Bag-1 binds the PDGF and HGF Receptor, and enhances growth factor-mediated protection from apoptosis. This represents a link between growth factor receptors and anti-apoptotic mechanisms. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Bag-1 by Western Blot. Western blot shows lysates of CTLL‑2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.6 µg/mL of Goat Anti-Mouse Bag-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF815) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Bag-1 at approximately 32 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6. | |
Immunoprecipitation of Mouse Bag‑1. Bag‑1 was immunoprecipitated from lysates (3 x 106 cells) of CTLL‑2 mouse cytotoxic T cell line and A431 human epithelial carcinoma cell line following incubation with 5 µg Goat Anti-Mouse Bag‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF815) for 1 hour at 4 ºC. Bag‑1-antibody complexes were absorbed using Protein G (Sigma). Immunoprecipitated Bag‑1 was detected by Western blot using 5 µg/mL Mouse Bag‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF815). The leftmost lane represents a control experiment where the antibody was incubated with no cell lysate. View our recommended buffer recipes for immunoprecipitation. |
