| 货号 | AF8119-SP |
| 别名 | afamin; AFM; ALB2; ALB2alpha-Alb; ALBA; ALBAalpha-albumin; ALF; Alpha-Alb; Alpha-albumin; MGC125338; MGC125339 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.2 µg/mL) |
| 目标/特异性 | Detects mouse Afamin in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human Afamin is observed. |
| 使用方法 | Western Blot: 0.2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 173 (Human); 280662 (Mouse); 282708 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Afamin Leu22-Met608 Accession # O89020 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Afamin, also known as AFM or alpha -Albumin,is an 84-88 kDa secreted monomeric glycoprotein member of theAlb/albumin family of molecules. Expressed by hepatocytes, CNSendothelial cells and osteoclasts, Afamin circulates in the blood atlow μg/mL concentrations. Afamin is known to bind and transportvitamin E, particularly under conditions where lipoprotein islimited. This is likely to be important in follicular fluid and CSF. Afamin also serves as an osteoclast-derived chemoattractant forpreosteoblasts, providing a rationale for the observation that boneformation often follows bone resorption. Mature mouse Afamin is 587amino acids (aa) in length (aa 22-608). It contains threeconsecutive albumin domains (aa 22-210, 211-403 and 404-599) thatcontain characteristic 5 or 6 intrachain disulfide bonds. Full-length mouse Afamin shares 67% aa sequence identity with humanAfamin. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Mouse Afamin by Western Blot. Western blot shows mouse serum. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse Afamin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8119) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Afamin at approximately 87 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
