| 货号 | AF7974-SP |
| 别名 | LCN13; Obp2a |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects mouse Lipocalin-13 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 227627 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Control of diabetic hyperglycaemia and insulin resistance through TSC22D4 | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Lipocalin-13 Ala19-Asp176 Accession # Q8K1H9 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | LCN-13 (Lipocalin 13; also Odorant-binding protein 2a) is a secreted, 26-28 kDa member of the LCN family, calycin superfamily of molecules. Its name is derived from Greek, and denotes the fact that lipocalins are known to be "fat-wrapping", or fat binding proteins that interact with small lipophilic molecules within a central cavity. LCN-13 is expressed by hepatocytes and smooth muscle cells. It is known to have antidiabetogenic effects, including the suppression of hepatic glucose production, the uptake of circulating glucose by adipocytes, and the induction of fatty acid oxidation by liver, possibly by stimulating CPT1 alpha production. Mouse LCN‑13 is synthesized as a 176 amino acid (aa) precursor that contains a 19 aa signal sequence and a 157 aa mature region. Although no splice forms have been reported for LCN-13 to date, a smaller, presumably proteolytically processed 23-26 kDa isoform has been noted on SDS-PAGE. Mature mouse LCN-13 shares 69% and 39% aa sequence identity with rat LCN-13 and human odorant-binding protein 2b, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Lipocalin-13 by Western Blot. Western blot shows lysates of mouse liver tissue, mouse skeletal muscle tissue, mouse epididymis tissue, and mouse mammary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Lipocalin-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7974) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lipocalin-13 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
