Mouse ICAM-1/CD54 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse ICAM-1/CD54 Affinity Purified Polyclonal Ab (25 UG)

货号： AF796-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF796-SP
别名	BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58
全称	Intercellular Adhesion Molecule 1
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse ICAM-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat ICAM-1 is observed, approximately 5% cross-reactivity with recombinant human (rh) ICAM-1 and rhICAM-3 is observed, and less than 1% cross-reactivity with recombinant mouse ICAM-2 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Adhesion Blockade: The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 10⁴ cells/well) to immobilized Recombinant Mouse ICAM-1 Fc Chimera (Catalog # 796-IC, 5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 5 µg/mL of the antibody.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3383 (Human); 15894 (Mouse); 25464 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Epidermal RAF prevents allergic skin disease Elife, 2016;5(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC - Paraffin embedded Abrogation of plasminogen activator inhibitor-1-vitronectin interaction ameliorates acute kidney injury in murine endotoxemia. Authors: Gupta K, Donahue D, Sandoval-Cooper M, Castellino F, Ploplis V PLoS ONE, 2015;10(3):e0120728. Species: Mouse Sample Type: Homogenized Tissue Application: WB Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice. Authors: Mathias D, Mitchel R, Barclay M, Wyatt H, Bugden M, Priest N, Whitman S, Scholz M, Hildebrandt G, Kamprad M, Glasow A PLoS ONE, 2015;10(3):e0119661. Species: Mouse Sample Type: Whole Tissue Application: IHC ICAM-1-based rabies virus vaccine shows increased infection and activation of primary murine B cells in vitro and enhanced antibody titers in-vivo. Authors: Norton, James E, Lytle, Andrew G, Shen, Shixue, Tzvetkov, Evgeni P, Dorfmeier, Corin L, McGettigan, James P PLoS ONE, 2014;9(1):e87098. Species: Hamster Sample Type: Cell Lysate Application: WB Peptide inhibitor of NF-kappaB translocation ameliorates experimental atherosclerosis. Authors: Mallavia B, Recio C, Oguiza A, Ortiz-Munoz G, Lazaro I, Lopez-Parra V, Lopez-Franco O, Schindler S, Depping R, Egido J, Gomez-Guerrero C Am J Pathol, 2013;182(5):1910-21. Species: Mouse Sample Type: Whole Tissue Application: IHC Fluorescence Skeletal muscle cells express ICAM-1 after muscle overload and ICAM-1 contributes to the ensuing hypertrophic response. Authors: Dearth C, Goh Q, Marino J, Cicinelli P, Torres-Palsa M, Pierre P, Worth R, Pizza F PLoS ONE, 2013;8(3):e58486. Species: Mouse Sample Type: Cell Lysate Application: WB Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma. Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M Biomark Insights, 2012;7(0):87-104. Species: Mouse Sample Type: BALF Application: WB Lung-targeted overexpression of the NF-kappaB member RelB inhibits cigarette smoke-induced inflammation. Authors: McMillan DH, Baglole CJ, Thatcher TH, Maggirwar S, Sime PJ, Phipps RP Am. J. Pathol., 2011;179(1):125-33. Species: Mouse Sample Type: Tissue Homogenates Application: WB Plasminogen activator inhibitor-1 regulates neutrophil influx during acute pyelonephritis. Authors: Roelofs JJ, Teske GJ, Bonta PI, de Vries CJ, Meijers JC, Weening JJ, van der Poll T, Florquin S Kidney Int., 2009;75(1):52-9. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Increased expression of CXCR4 and integrin alphaM in hypoxia-preconditioned cells contributes to improved cell retention and angiogenic potency. Authors: Kubo M, Li TS, Kamota T, Ohshima M, Qin SL, Hamano K J. Cell. Physiol., 2009;220(2):508-14. Species: Mouse Sample Type: Cell Lysates Application: WB Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection. Authors: Sofra V, Mansour S, Liu M, Gao B, Primpidou E, Wang P, Li S Eur. J. Immunol., 2009;39(1):85-95. Species: Mouse Sample Type: Tissue Homogenates Application: WB The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion. Authors: Kato N, Yuzawa Y, Kosugi T J. Am. Soc. Nephrol., 2009;20(7):1565-76. Species: Mouse Sample Type: Tissue Homogenates Application: WB Apelin prevents aortic aneurysm formation by inhibiting macrophage inflammation. Authors: Leeper NJ, Tedesco MM, Kojima Y, Schultz GM, Kundu RK, Ashley EA, Tsao PS, Dalman RL, Quertermous T Am. J. Physiol. Heart Circ. Physiol., 2009;296(5):H1329-35. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded PPARgamma agonist rosiglitazone is neuroprotective after traumatic brain injury via anti-inflammatory and anti-oxidative mechanisms. Authors: Yi JH, Park SW, Brooks N, Lang BT, Vemuganti R Brain Res., 2008;1244(0):164-72. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Atherosclerosis prevention by a fish oil-rich diet in apoE(-/-) mice is associated with a reduction of endothelial adhesion molecules. Authors: Casos K, Saiz MP, Ruiz-Sanz JI, Mitjavila MT Atherosclerosis, 2008;201(2):306-17. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Vascular inflammation, insulin resistance, and reduced nitric oxide production precede the onset of peripheral insulin resistance. Authors: Kim F, Pham M, Maloney E, Rizzo NO, Morton GJ, Wisse BE, Kirk EA, Chait A, Schwartz MW Arterioscler. Thromb. Vasc. Biol., 2008;28(11):1982-8. Species: Mouse Sample Type: Tissue Homogenates Application: WB Dietary alpha-lipoic acid supplementation inhibits atherosclerotic lesion development in apolipoprotein E-deficient and apolipoprotein E/low-density lipoprotein receptor-deficient mice. Authors: Zhang WJ, Bird KE, McMillen TS, LeBoeuf RC, Hagen TM, Frei B Circulation, 2007;117(3):421-8. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded High pressure promotes monocyte adhesion to the vascular wall. Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S Circ. Res., 2007;100(8):1226-33. Species: Mouse Sample Type: Whole Tissue Application: IHC Fractalkine upregulates intercellular adhesion molecule-1 in endothelial cells through CX3CR1 and the Jak Stat5 pathway. Authors: Yang XP, Mattagajasingh S, Su S, Chen G, Cai Z, Fox-Talbot K, Irani K, Becker LC Circ. Res., 2007;101(10):1001-8. Species: Mouse Sample Type: Tissue Homogenates Application: WB Crosstalk between keratinocytes and adaptive immune cells in an IkappaBalpha protein-mediated inflammatory disease of the skin. Authors: Rebholz B, Haase I, Eckelt B, Paxian S, Flaig MJ, Ghoreschi K, Nedospasov SA, Mailhammer R, Debey-Pascher S, Schultze JL, Weindl G, Forster I, Huss R, Stratis A, Ruzicka T, Rocken M, Pfeffer K, Schmid RM, Rupec RA Immunity, 2007;27(2):296-307. Species: Mouse Sample Type: Whole Tissue Application: IHC Activation of mouse protease-activated receptor-2 induces lymphocyte adhesion and generation of reactive oxygen species. Authors: Lim SY, Tennant GM, Kennedy S, Wainwright CL, Kane KA Br. J. Pharmacol., 2006;149(5):591-9. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG J. Exp. Med., 2006;203(12):2763-77. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Authors: Mendez MP, Morris SB, Wilcoxen S, Greeson E, Moore B, Paine R Am. J. Physiol. Lung Cell Mol. Physiol., 2006;290(5):L962-70. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse ICAM-1/CD54 Gln28-Asn485 Accession # Q3U8M7
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.
运输条件	Blue Ice
存放说明	4℃
参考文献	Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74. Siu, G. et al. (1989) J. Immunol. 143:3813. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

参考图片

ICAM‑1/CD54 in Mouse Testis. ICAM‑1/CD54 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF796) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in Leydig cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.