| 货号 | AF7818-SP |
| 别名 | AW554172; GP55; GP55Stromal cell-derived receptor 1; GP65SDR-1; GP66; MGC102805; neuroplastin; np55; np65; NPTN; SDFR1; SDFR1DKFZp686L2477; SDR1; SDR1stromal cell derived factor receptor 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.05 µg/mL) |
| 目标/特异性 | Detects mouse Neuroplastin in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant human Neuroplastin-65 is observed. |
| 使用方法 | Western Blot: 0.05 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 27020 (Human); 20320 (Mouse); 56064 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Neuroplastin Gln29-Pro338, predicted Accession # P97300 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Neuroplastin (NPTN or neural plasticity-dependent synaptic rearrangement protein; also Np65 and SDR-1) is a 63-67 kDa member of the CD147 family, Ig‑superfamily of molecules. Its expression is primarily restricted to the PSD of select neurons, and it likely serves as a cell adhesion molecule between cells. NPTN is known to bind homotypically in trans, and this complex appears to interact with FGFR1 in cis. Engagement of the FGFR induces neurite outgrowth. Notably, rodent and human Np65 show distinctly different staining patterns in the hippocampus and cerebellum of the brain. Mature mouse NPTN is a 369 amino acid (aa) type I transmembrane glycoprotein. It possesses a 310 aa extracellular region (aa 29-338) that contains three Ig-like domains (aa 29-134; 148-234; 237-327), and a 38 aa cytoplasmic tail. There are three splice forms that have been described. One is a widely-expressed 53-57 kDa isoform (Np55) that contains a Glu substitution for aa 31‑147, a second is a splice form that shows the above substitution coupled to a deletion of aa 371-374, and a third is an isoform that contains a nine aa substitution for aa 1-235. Over aa 1-338, mouse NPTN shares 95% and 98% aa sequence identity with human and rat NPTN/Np65, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Neuroplastin by Western Blot. Western blot shows lysates of mouse brain (whole) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 0.05 µg/mL of Sheep Anti-Mouse Neuroplastin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7818) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neuroplastin at approximately 55-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
