| 货号 | AF776-SP |
| 别名 | IBP6; IBP-6; IGF binding protein 6; IGF-binding protein 6; IGFBP-6; insulin-like growth factor binding protein 6; insulin-like growth factor-binding protein 6 | 全称 | Insulin-like Growth Factor Binding Protein 6 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse IGFBP-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) IGFBP-6 is observed and less than 1% cross-reactivity with rhIGFBP-1, rhIGFBP-2, rhIGFBP-3, rhIGFBP-4, and rhIGFBP-5 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3489 (Human); 16012 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Myelin formation during development of the CNS is delayed in matrix metalloproteinase-9 and -12 null mice. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-6 Gly24-Gly238 Accession # P47880 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Mouse IGFBP-6 cDNA encodes a 238 amino acid (aa) residue precursor protein with a putative 25 aa residue signal peptide that is processed to generate the 213 aa residue mature protein that is O-glycosylated. Mouse and human IGFBP-6 share 73% amino acid similarity. Mouse and rat IGFBP-6 share 94% amino acid similarity and the mouse IGFBP-6 has a 9 amino acid insertion compared to the rat homolog. IGFBP-6 is expressed in ovarian, testicular, muscle, heart and lung tissues in the adult mouse. IGFBP-6 was not detected in total RNA from a whole mouse embryo. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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