Mouse EMMPRIN/CD147 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse EMMPRIN/CD147 Affinity Purified Polyclonal Ab (25 UG)

货号： AF772-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF772-SP
别名	5F7; basigin (Ok blood group); Basigin; BSG; CD147 antigen; CD147; Collagenase stimulatory factor; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK blood group antigen; OK; Tumor cell-derived collagenase stimulatory factor
全称	Extracellular Matrix Metalloproteinase Inducer
反应种属	Mouse
应用	Western Blot(0.2 µg/mL) Simple Western(2 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects mouse EMMPRIN/CD147 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human EMMPRIN is observed.
使用方法	Western Blot: 0.2 µg/mL Simple Western: 2 µg/mL Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	682 (Human); 12215 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Basigin/CD147 promotes renal fibrosis after unilateral ureteral obstruction. Authors: Kato N, Kosugi T, Sato W, Ishimoto T, Kojima H, Sato Y, Sakamoto K, Maruyama S, Yuzawa Y, Matsuo S, Kadomatsu K Am. J. Pathol., 2011;178(2):572-9. Species: Mouse Sample Type: Tissue Homogenates Application: WB The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion. Authors: Kato N, Yuzawa Y, Kosugi T J. Am. Soc. Nephrol., 2009;20(7):1565-76. Species: Mouse Sample Type: Tissue Homogenates Application: WB Basigin-mediated gene expression changes in mouse uterine stromal cells during implantation. Authors: Chen L, Belton RJ, Nowak RA Endocrinology, 2009;150(2):966-76. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Evaluation of adenovirus-delivered human CD59 as a potential therapy for AMD in a model of human membrane attack complex formation on murine RPE. Authors: Ramo K, Cashman SM, Kumar-Singh R Invest. Ophthalmol. Vis. Sci., 2008;49(9):4126-36. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse EMMPRIN/CD147 Ala22-Arg209 Accession # NP_001070652
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily.
运输条件	Blue Ice
存放说明	4℃
参考文献	Aspartic Proteases and Regulators B Cell Chemotaxis, Migration, and Adhesion B Cell Markers Cancer Biomarkers Dendritic Cell Adhesion and Migration Human CD Marker Products ICC / IHC Images: EMMPRIN/CD147 Metalloproteases and Regulators MMPs, TIMPs and Related Molecules Platelets T Cell Migration/Adhesion

参考图片

Detection of Mouse EMMPRIN/CD147 by Western Blot. Western blot shows lysates of mouse brain tissue, mouse heart tissue, and mouse small intestine tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF772) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EMMPRIN/CD147 at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse EMMPRIN/CD147 by Simple Western^TM. Simple Western lane view shows lysates of mouse brain (cortex) tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for EMMPRIN/CD147 at approximately 62 kDa (as indicated) using 2 µg/mL of Goat Anti-Mouse EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF772) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.