Mouse IL-1 RI Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-1 RI Affinity Purified Polyclonal Ab (25 UG)

货号： AF771-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF771-SP
别名	CD121 antigen-like family member A; CD121a antigen; CD121a; IL1R; IL1R1; IL1RT1; IL-1RT1; IL-1RT-1; Interleukin 1 Receptor 1; interleukin 1 receptor, type I; interleukin receptor 1; interleukin-1 receptor type 1; p80
全称	Interleukin 1 Receptor I
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse IL-1 RI in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) IL-18 R is observed and 5% cross-reactivity with rmIL-18 R beta, recombinant human IL-1 RI, and recombinant rat IL-1 Rrp2 is observed and less than 1% cross-reactivity with rmIL-1 RII is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 0.2-1.0 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 alpha /IL‑1F1 and 1.25 µg/mL concanavalin A.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3554 (Human); 16177 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Bone marrow-derived microglia infiltrate into the paraventricular nucleus of chronic psychological stress-loaded mice. Authors: Ataka K, Asakawa A, Nagaishi K, Kaimoto K, Sawada A, Hayakawa Y, Tatezawa R, Inui A, Fujimiya M PLoS ONE, 2013;8(11):e81744. Species: Mouse Sample Type: tissue Application: IHC Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo. Authors: Proebstl D, Voisin MB, Woodfin A, Whiteford J, DAcquisto F, Jones GE, Rowe D, Nourshargh S J. Exp. Med., 2012;209(6):1219-34. Species: Mouse Sample Type: Whole Tissue Application: IHC Interleukin-1beta causes long-term potentiation deficiency in a mouse model of septic encephalopathy. Authors: Imamura Y, Wang H, Matsumoto N, Muroya T, Shimazaki J, Ogura H, Shimazu T Neuroscience, 2011;187(0):63. Species: Mouse Sample Type: Tissue Homogenates Application: WB Changes in melanocortin expression and inflammatory pathways in fetal offspring of nonhuman primates fed a high-fat diet. Authors: Grayson BE, Levasseur PR, Williams SM, Smith MS, Marks DL, Grove KL Endocrinology, 2010;151(4):1622-32. Species: primate - Macaca fuscata (Japanese macaque) Sample Type: Whole Tissue Application: IHC Frozen Type 2 diabetes impairs insulin receptor substrate-2-mediated phosphatidylinositol 3-kinase activity in primary macrophages to induce a state of cytokine resistance to IL-4 in association with overexpression of suppressor of cytokine signaling-3. Authors: O&amp;apos;Connor JC, Sherry CL, Guest CB, Freund GG J. Immunol., 2007;178(11):6886-93. Species: Mouse Sample Type: Peritoneal Lavage Application: ELISA Development IL-1beta induces a MyD88-dependent and ceramide-mediated activation of Src in anterior hypothalamic neurons. Authors: Davis CN, Tabarean I, Gaidarova S, Behrens MM, Bartfai T J. Neurochem., 2006;98(5):1379-89. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse IL-1 RI Leu20-Lys338 Accession # P13504
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex. Mouse IL-1 RI shares 63% amino acid sequence homology with human IL-1 RI in their extracellular domains. An IL-1 receptor accessory protein (1) that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta  but not IL-1ra, was identified. This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.
运输条件	Blue Ice
存放说明	4℃
参考文献	Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

参考图片

Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Mouse IL‑1 RI Antibody. Recombinant Mouse IL‑1 alpha /IL‑1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 alpha /IL‑1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF771). The ND₅₀ is typically 0.2-1.0 µg/mL in the presence of concanavalin A (1.25 µg/mL).