| 货号 | AF7688-SP |
| 别名 | EC 3.4.21; EC 3.4.21.117; hK7; hSCCE; kallikrein 7 (chymotryptic, stratum corneum); kallikrein-related peptidase 7; KLK7; protease, serine, 6; PRSS6; SCCEkallikrein-7; Serine protease 6; signal protein; Stratum corneum chymotryptic enzyme |
| 反应种属 | Mouse |
| 应用 | Western Blot(2 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Kallikrein 7 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Kallikrein 7 is observed, and less than 1% cross-reactivity with recombinant mouse Kallikrein 5 is observed. |
| 使用方法 | Western Blot: 2 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5650 (Human); 23993 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Kallikrein 7 Gln22-Arg249 (predicted) Accession # Q91VE3 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | KLK7 (Kallikrein 7; also Serine protease 6, Thymopsin and Stratum corneum chymotryptic enzyme/SCCE) is a 27 kDa (predicted), secreted glycoprotein member of the kallikrein subfamily, peptidase S1 family of enzymes. It is an IL-4 and IL-13-inducible chymotrypsin-like serine protease that has restricted expression, being associated with stratum spinosum and granulosum keratinocytes, distal and proximal convoluted tubule epithelium, limited pancreatic acinar cells. In contrast, KLK7 is widely expressed by a variety of carcinoma cell types. KLK7 preferentially cleaves peptide bonds that contain an aromatic (Tyr or Phe) residue in the P1 (or postcleavage C-terminal amino acid) position. Molecules known to serve as substrates for KLK7 include chemerin, fibronectin, E-cadherin, LL37, procaspase-14, MMP-9 and desmoglein. The nature of the KLK7 substrates indicates that it plays a key role in intercellular adhesion dissolution with subsequent cell migration. Mouse KLK7 is synthesized as a 249 amino acid (aa) precursor. It contains a 21 aa signal sequence, a four aa prosegment, and a 224 aa mature region (aa 26-249). The mature molecule contains one large protease domain (aa 26-246) and a variable, three aa C-terminus that possesses either a VSW or ASR motif. Over aa 22-249, mouse KLK7 shares 75% and 85% aa sequence identity with human and hamster KLK7, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Mouse Kallikrein 7 by Western Blot. Western blot shows lysates of mouse skin tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7688) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Kallikrein 7 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Kallikrein 7 in Mouse Skin. Kallikrein 7 was detected in perfusion fixed frozen sections of mouse skin using Sheep Anti-Mouse Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7688) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to keratinocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
