Mouse TCF-3/E2A Affinity Purified Polyclonal Ab (25 UG)
货号：
AF7650-SP 基本售价：
1378.1 元 规格：
-
产品信息
概述| 货号 | AF7650-SP |
| 别名 | bHLHb21; bHLHb21TCF-3; Class B basic helix-loop-helix protein 21; E2A immunoglobulin enhancer-binding factor E12/E47; E2A; E2AKappa-E2-binding factor; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; ITF1; ITF1Transcription factor ITF-1; Kappa-E2-binding factor; MGC129647; MGC129648; Pan2; Tcfe2a; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); Transcription factor 3; transcription factor E2-alpha; VDIR; VDR interacting repressor |
全称 | Transcription Factor 3/E2-alpha | | 反应种属 | Mouse |
| 应用 | Western Blot(0.5 µg/mL)
|
| 目标/特异性 | Detects mouse TCF-3/E2A in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL
|
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 6929 (Human); 21423 (Mouse); 312451 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse TCF-3/E2A
Asn33-Arg159
Accession # P15806 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | TCF-3 (Transcription factor 3; also Transcription factor A1 and E2A/alpha plus E12/E47) is a 68-75 kDa nuclear class I member of the bHLH family of molecules. It should not be confused with TCF-3, otherwise known as TCF7-L1, an epidermis-associated beta -catenin regulator. TCF-3 is ubiquitously expressed, and generally serves as a transcriptional regulator. It is able to form covalent homodimers, and following phosphorylation, heterodimers with tissue-specific class II bHLH factors, thus impacting the proliferation and differentiation of select cell types. While heterodimers, and possibly homodimers, activate genes by binding to E boxes, heterodimers involving Id proteins are gene repressors. Mouse TCF-3/E12 is 651 amino acids (aa) in length. It contains one Leu-zipper motif (aa 387-422) plus a bHLH domain (aa 544‑604). TCF-3 contains at least three potential Ser phosphorylation sites. There is one alternative splice form that is termed E47 and shows a 69 aa substitution for aa 527-598. This involves the bHLH domain, and it is believed that each splice form has a distinct function(s). Both splice forms (E12 and E47) do occur simultaneously in the same cell type. Over aa 33-159, mouse TCF-3 shares 94% and 75% aa sequence identity with rat and human TCF-3, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - B Cell Development and Differentiation
- Cancer Biomarkers
- Epithelial Stem Cell Transcription Factors
- HIF Transcription Factors
- Homeodomain (Hox) Transcription Factors
- ICC / IHC Images: TCF-3/E2A
- Neural Stem Cell Transcription Factors
- Pluripotent Stem Cell Transcription Factors
- T Cell Antigen Recognition
- Wnt Intracellular Signaling
|
参考图片Detection of Mouse TCF‑3/E2A by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line, P19 mouse embryonal carcinoma cell line, C2C12 mouse myoblast cell line, and beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse TCF‑3/E2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7650) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for TCF‑3/E2A at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
