| 货号 | AF7649-SP |
| 别名 | antigen identified by monoclonal Ki-67; antigen KI-67; Ki67; Ki-67; KIA; Marker Of Proliferation Ki-67; MIB-1; MKI67; PPP1R105; proliferation-related Ki-67 antigen; Protein Phosphatase 1; Regulatory Subunit 105; TSG126 | 全称 | Antigen Identified by Monoclonal Antibody Ki-67 |
| 反应种属 | Mouse |
| 应用 | Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Ki-67/MKI67 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human Ki-67/MKI67 is observed. |
| 使用方法 | Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4288 (Human); 17345 (Mouse); 291234 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Ki-67/MKI67 Asn3053-Ser3177 Accession # NP_001074586 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | MKI67 (also Ki-67 and TSG126) is a 350-370 kDa nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins. Ki-67 was originally recognized as an antigen associated with the monoclonal Ki-67 antibody raised against Hodgkins lymphoma nuclear material. Ki-67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle. Thus, MKI67 qualifies as a cell proliferation marker. Functionally, Ki-67 is known to interact with 160 kDa Hklp2, a protein that promotes centrosome separation and spindle bipolarity. It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis. Mouse MKI67 is 3177 amino acids (aa) in length. It contains one FHA domain (aa 8-101), followed by sixteen 120 aa repeats (aa 993-2872). There are two potential isoform variants. One isoform shows a 19 aa substitution for 1120-3177, while a second isoform contains a deletion of aa 1169-1409. Over aa 3053-3177, mouse Ki-67 shares 46% and 74% aa sequence identity with the human and rat orthologs to Ki-67, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Ki-67/MKI67 in NIH‑3T3 Mouse Cell Line. Ki-67/MKI67 was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line using Sheep Anti-Mouse Ki-67/MKI67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7649) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and nucleoli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
