| 货号 | AF7608-SP |
| 别名 | baculoviral IAP repeat-containing 1; BIRC1; BIRC1baculoviral IAP repeat-containing protein 1; FLJ18088; FLJ42520; FLJ58811; Neuronal apoptosis inhibitory protein; NLR family, apoptosis inhibitory protein; NLR family, BIR domain containing 1; NLRB1; nucleotide-binding oligomerization domain, leucine rich repeat and BIR domaincontaining 1; psi neuronal apoptosis inhibitory protein; psiNAIP | 全称 | Neuronal Apoptosis Inhibitor Protein |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse NAIP in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse NAIP2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4671 (Human); 17940 (Mouse); 191568 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse NAIP Lys783-Ser856 Accession # Q9QWK5 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | NAIP-1 (Neuronal Apoptosis Inhibitory Protein 1; also Naip and Birc1) is a 150-160 kDa member of both the NLR/PRR and IAP family of proteins. It is widely, but not ubiquitously expressed, and is found in adipocytes, macrophages, neurons, renal proximal and distal convoluted tubules, and nonproliferative intestinal epithelium. NAIP-1 plays a key regulatory role in caspase activation. It promotes caspase-1 activation, blocks caspase-3 and -7 activation, and by binding neuronal hippocalcin, inhibits caspase-3-independent apoptosis. Mouse NAIP-1 is 1403 amino acids (aa) in length. It contains three consecutive N-terminal BIR (Baculovirus Inhibition of apoptosis Repeat) domains (aa 63-346) and one NACHT region (aa 464-758). The BIR #2 domain is posited to bind both caspase-3 and -7, while the BIR domain #3 likely interacts with hippocalcin. NAIP-1 undergoes proteolysis, generating an N-terminal 100 kDa fragment (likely aa 1- ≈ 869) and a 60 kDa C-terminal fragment (likely aa 870-1403). The C-terminal fragment undergoes further processing to generate a 25 kDa and 35 kDa fragment. There are at least seven numbered NAIP genes in mouse that have arisen by gene duplication. Over the aa range 783-856, they share less than 60% aa sequence identity with NAIP-1. Over the same aa range, mouse NAIP-1 shares 54% aa sequence identity with the only human NAIP gene. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse NAIP by Western Blot. Western blot shows lysates of mouse liver tissue and mouse colon tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse NAIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7608) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NAIP at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
