| 货号 | AF7490-SP |
| 别名 | ARVD11; Cadherin family member 2; CDHF2; CDHF2DGII/III; desmocollin 2; desmocollin-2; Desmocollin-3; Desmosomal glycoprotein II; desmosomal glycoprotein II/III; Desmosomal glycoprotein III; DG2; DSC2; DSC3DKFZp686I11137 |
| 反应种属 | Mouse |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Desmocollin-2 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Desmocollin-2 is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Desmocollin-1 and rmDesmocollin-3 is observed. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1824 (Human); 13506 (Mouse); 291760 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Desmocollin-2 Arg136-Pro694 Accession # P55292 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | DSC-2 (Desmocollin [Greek for "glue-that-binds"]-2; also epithelial type 2 desmocollin) is a 100-120 kDa member of the Ca++-dependent cadherin family of adhesion molecules. It is found on the surface of both simple and stratified epithelium that form desmosomes. DSC-2 expression is induced by DSG-2 where it serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. Homodimeric DSC-2 forms in-trans, and DSC-2 may also form heterotypic interactions with DSG-2. A DSC-2:DSG-2 cis-interaction is suggested to block DSG-2 activation of Akt, an action that results in beta -catenin mediated cell proliferation. Mature mouse DSC-2 is a 767 amino acid (aa) type I transmembrane glycoprotein (aa 136-902). The mature molecule contains a 559 aa extracellular region (aa 136-694) with five cadherin domains (aa 136-694) and a 187 aa cytoplasmic tail (aa 716-902). There is one 100-110 kDa splice variant that shows an 11 aa substitution for aa 838-902. Over aa 136-694, mouse DSC-2 shares 87% and 79% aa sequence identity with rat and human DSC-2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Desmocollin‑2 in Mouse Embryo. Desmocollin‑2 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse Desmocollin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7490) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
