Mouse VEGF R3/Flt-4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse VEGF R3/Flt-4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF743-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF743-SP
别名	EC 2.7.10; EC 2.7.10.1; FLT4; Flt-4; fms-related tyrosine kinase 4; LMPH1A; PCLFLT41; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; Tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor receptor 3; VEGFR-3; VEGFR3Fms-like tyrosine kinase 4
全称	Vascular Endothelial Growth Factor Receptor 3
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects mouse VEGF R3/Flt-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VEGF R3, 10% cross-reactivity with rhVEGF R2, and 5% cross-reactivity with rhVEGF R1.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2324 (Human); 14257 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves Authors: Boksik Cha Genes Dev, 2016;30(12):1454-69. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Structural and functional features of central nervous system lymphatic vessels. Authors: Louveau A, Smirnov I, Keyes T, Eccles J, Rouhani S, Peske J, Derecki N, Castle D, Mandell J, Lee K, Harris T, Kipnis J Nature, 2015;523(7560):337-41. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified Delayed Healing of Sickle Cell Ulcers Is due to Impaired Angiogenesis and CXCL12 Secretion in Skin Wounds. Authors: Nguyen V, Nassar D, Batteux F, Raymond K, Tharaux P, Aractingi S J Invest Dermatol, 2015;136(2):497-506. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded A dural lymphatic vascular system that drains brain interstitial fluid and macromolecules. Authors: Aspelund A, Antila S, Proulx S, Karlsen T, Karaman S, Detmar M, Wiig H, Alitalo K J Exp Med, 2015;212(7):991-9. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina. Authors: Zhou Y, Williams J, Smallwood P, Nathans J PLoS ONE, 2015;10(12):e0143650. Species: Mouse Sample Type: tissue Application: IHC-Frozen Quantitative assessment of angiogenesis, perfused blood vessels and endothelial tip cells in the postnatal mouse brain. Authors: Walchli T, Mateos J, Weinman O, Babic D, Regli L, Hoerstrup S, Gerhardt H, Schwab M, Vogel J Nat Protoc, 2015;10(1):53-74. Species: Mouse Sample Type: Whole Tissue Application: IHC Endothelial Notch activity promotes angiogenesis and osteogenesis in bone. Authors: Ramasamy S, Kusumbe A, Wang L, Adams R Nature, 2014;507(7492):376-80. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Preferential lymphatic growth in bronchus-associated lymphoid tissue in sustained lung inflammation. Authors: Baluk P, Adams A, Phillips K, Feng J, Hong Y, Brown M, McDonald D Am J Pathol, 2014;184(5):1577-92. Species: Mouse Sample Type: Whole Tissue Application: IHC Lenalidomide inhibits lymphangiogenesis in preclinical models of mantle cell lymphoma. Authors: Song K, Herzog B, Sheng M, Fu J, McDaniel J, Chen H, Ruan J, Xia L Cancer Res, 2013;73(24):7254-64. Species: Mouse Sample Type: Tissue Lysate Application: WB Proteinuria triggers renal lymphangiogenesis prior to the development of interstitial fibrosis. Authors: Yazdani S, Poosti F, Kramer A, Mirkovic K, Kwakernaak A, Hovingh M, Slagman M, Sjollema K, de Borst M, Navis G, van Goor H, van den Born J PLoS ONE, 2012;7(11):e50209. Species: Mouse Sample Type: Whole Tissue Application: IHC Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling. Authors: Benedito R, Rocha S, Woeste M, Zamykal M, Radtke F, Casanovas O, Duarte A, Pytowski B, Adams R Nature, 2012;484(7392):110-4. Species: Mouse Sample Type: Cell Lysate Application: ELISA Development Remodeling of the lymphatic vasculature during mouse mammary gland morphogenesis is mediated via epithelial-derived lymphangiogenic stimuli. Authors: Betterman K, Paquet-Fifield S, Asselin-Labat M, Visvader J, Butler L, Stacker S, Achen M, Harvey N Am J Pathol, 2012;181(6):2225-38. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Neuropilin-2 mediates VEGF-C-induced lymphatic sprouting together with VEGFR3. Authors: Xu Y, Yuan L, Mak J, Pardanaud L, Caunt M, Kasman I, Larrivee B, Del Toro R, Suchting S, Medvinsky A, Silva J, Yang J, Thomas JL, Koch AW, Alitalo K, Eichmann A, Bagri A J. Cell Biol., 2010;188(1):115-30. Species: Mouse Sample Type: Whole Tissue Application: IHC Fresh Vascular endothelial growth factor-C induces lymphangitic carcinomatosis, an extremely aggressive form of lung metastases. Authors: Das S, Ladell DS, Podgrabinska S Cancer Res., 2010;70(5):1814-24. Species: Mouse Sample Type: Whole Tissue Application: IHC Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis. Authors: Wang Y, Nakayama M, Pitulescu ME, Schmidt TS, Bochenek ML, Sakakibara A, Adams S, Davy A, Deutsch U, Luthi U, Barberis A, Benjamin LE, Makinen T, Nobes CD, Adams RH Nature, 2010;465(7297):483-6. Species: Mouse Sample Type: Cell Lysates Application: IP Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development. Authors: Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY J. Clin. Invest., 2010;120(5):1694-707. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Blocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation. Authors: Tammela T, Zarkada G, Wallgard E, Murtomaki A, Suchting S, Wirzenius M, Waltari M, Hellstrom M, Schomber T, Peltonen R, Freitas C, Duarte A, Isoniemi H, Laakkonen P, Christofori G, Yla-Herttuala S, Shibuya M, Pytowski B, Eichmann A, Betsholtz C, Alitalo K Nature, 2008;454(7204):656-60. Species: Mouse Sample Type: Whole Tissue Application: IHC Fresh Transgenic induction of vascular endothelial growth factor-C is strongly angiogenic in mouse embryos but leads to persistent lymphatic hyperplasia in adult tissues. Authors: Lohela M, Helotera H, Haiko P, Dumont DJ, Alitalo K Am. J. Pathol., 2008;173(6):1891-901. Species: Mouse Sample Type: Whole Tissue Application: IHC Vascular endothelial growth factor receptor 3 is involved in tumor angiogenesis and growth. Authors: Laakkonen P, Waltari M, Holopainen T, Takahashi T, Pytowski B, Steiner P, Hicklin D, Persaud K, Tonra JR, Witte L, Alitalo K Cancer Res., 2007;67(2):593-9. Species: Mouse Sample Type: Whole Tissue Application: IHC Distinct vascular endothelial growth factor signals for lymphatic vessel enlargement and sprouting. Authors: Wirzenius M, Tammela T, Uutela M, He Y, Odorisio T, Zambruno G, Nagy JA, Dvorak HF, Yla-Herttuala S, Shibuya M, Alitalo K J. Exp. Med., 2007;204(6):1431-40. Species: Mouse Sample Type: Whole Tissue Application: IHC VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain. Authors: Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Breant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL Nat. Neurosci., 2006;9(3):340-8. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Visualization of IL-12/23p40 in vivo reveals immunostimulatory dendritic cell migrants that promote Th1 differentiation. Authors: Reinhardt RL, Hong S, Kang SJ, Wang ZE, Locksley RM J. Immunol., 2006;177(3):1618-27. Species: Mouse Sample Type: Whole Tissue Application: IHC Desmoplakin and Plakoglobin--specific markers of lymphatic vessels in the skin? Authors: Fedele C, Berens D, Rautenfeld V, Pabst R Anat Histol Embryol, 2004;33(3):168-71. Species: Equine Sample Type: Whole Tissue Application: IHC B lymphocyte-specific c-Myc expression stimulates early and functional expansion of the vasculature and lymphatics during lymphomagenesis. Authors: Ruddell A, Mezquita P, Brandvold KA, Farr A, Iritani BM Am. J. Pathol., 2003;163(6):2233-45. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF R3/Flt-4 Tyr25-Asp770 Accession # P35917
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	VEGF R3 (Flt-4), together with VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. In adults, VEGF R3 expression is restricted to the endothelial cells of the lymphatic vessels. Mouse VEGF R3 cDNA encodes a 1363 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGF R3 has a 751 aa residue extracellular domain, a 22 aa residue hydrophobic transmembrane domain and a 565 aa residue cytoplasmic domain. The polypeptide sequences of murine Flt-4 is 88% identical to the human homologue. VEGF R3 has been reported to serve as the receptors for VEGF-C and VEGF-D.
运输条件	Blue Ice
存放说明	4℃
参考文献	Finnerty, H.et al. (1993) Oncogene 8:2293. Joukov, V.et al. (1996) EMBO J. 15:290. Achen, M.et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.

参考图片

Detection of VEGF R3/Flt‑4 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 cells, a mouse endothelioma cell line, was stained with Goat Anti-Mouse VEGF R3/Flt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF743, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by PE-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).