Mouse B7-1/CD80 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse B7-1/CD80 Affinity Purified Polyclonal Ab (25 UG)

货号： AF740-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF740-SP
别名	Activation B7-1 antigen; B7; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80
反应种属	Mouse
应用	Western Blot,Flow Cytometry,CyTOF-ready,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects mouse B7‑1/CD80 in ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize B7‑1/CD80-induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND₅₀) is typically 0.15-0.6 µg/mL in the presence of 0.1 µg/mL Recombinant Mouse B7‑1/CD80 Fc Chimera and 10 µg/mL PHA.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection. Authors: Sofra V, Mansour S, Liu M, Gao B, Primpidou E, Wang P, Li S Eur. J. Immunol., 2009;39(1):85-95. Species: Mouse Sample Type: Tissue Homogenates Application: WB Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract. Authors: He H, Li W, Chen SY, Zhang S, Chen YT, Hayashida Y, Zhu YT, Tseng SC Invest. Ophthalmol. Vis. Sci., 2008;49(10):4468-75. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse B7‑1/CD80 Asp37-Lys245 Accession # Q00609
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Mouse B7-1 is a 306 amino acid (aa) protein containing a putative 37 aa signal peptide, a 190 aa extracellular domain, a 22 aa transmembrane domain, and a 38 aa cytoplasmic domain. Mouse B7-1 and B7-2 share 28% amino acid identity. Mouse and human B7-1 share 44% amino acid identity. However, it has been observed that both human and mouse B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.
运输条件	Blue Ice
存放说明	4℃
参考文献	Azuma, M.et al. (1993) Nature 366:76. Freeman, G.J.et al. (1993) Science 262:909. Freeman, G.et al. (1991) J. Exp. Med. 174:625. Selvakumar, A.et al. (1993) Immunogenetics 38:292. Chen, C.et al. (1994) J. Immunol. 152:4929. Freeman, G.J.et al. (1993) J. Exp. Med. 178:2185.

参考图片

Detection of Mouse B7‑1/CD80 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for B7‑1/CD80 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of B7‑1/CD80 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either treated with 200 ng/mL LPS (filled histogram) or unstimulated (open histogram) were stained with Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

B7‑1/CD80 in Mouse Splenocytes.
B7‑1/CD80 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

IL‑2 secretion Induced by
B7‑1/CD80 and Neutralization by Mouse
B7‑1/CD80 Antibody. Recombinant Mouse
B7‑1/CD80 Fc Chimera (Catalog # 740-B1) co-stimulates IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). IL‑2 secretion elicited by Recombinant Mouse
B7‑1/CD80 Fc Chimera (0.1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740). The ND₅₀ is typically
0.15‑0.6 µg/mL.