| 货号 | AF737-SP |
| 别名 | CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; selectin P (granule membrane protein 140kDa, antigen CD62); SELP |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects mouse P-Selectin/CD62P in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human P-Selectin/CD62P is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6403 (Human); 20344 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Reduced plaque formation induced by rosiglitazone in an STZ-diabetes mouse model of atherosclerosis is associated with downregulation of adhesion molecules. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse P-Selectin/CD62P Trp42-Ala709 Accession # Q01102 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mouse P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1). P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. Mouse P-Selectin cDNA encodes a 768 amino acid (aa) residue type I transmembrane protein with a 41 aa signal peptide, a 668 aa extracellular domain, a transmembrane domain and a short (35 aa) cytoplasmic domain. The extracellular domain has an NH2-terminal C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains. The extracellular domains of human and mouse P-Selectin share approximately 73% sequence homology. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Rat P‑Selectin/CD62P by Simple WesternTM. Simple Western lane view shows lysates of rat platelets, loaded at 0.2 mg/mL. A specific band was detected for P‑Selectin/CD62P at approximately 197 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse P‑Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. | |
Detection of Mouse and Rat P‑Selectin/CD62P by Western Blot. Western blot shows lysates of mouse platelets and rat platelets. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse/Rat P‑Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for P‑Selectin/CD62P at approximately 140-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
