| 货号 | AF7335-SP |
| 别名 | CD90 antigen; CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 membrane glycoprotein; Thy-1 T-cell antigen; Thy1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.2 µg/mL) |
| 目标/特异性 | Detects mouse CD90/Thy1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human CD90/Thy1 is observed. |
| 使用方法 | Western Blot: 0.2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7070 (Human); 21838 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse CD90/Thy1 Gln20-Lys130 Accession # P01831 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | CD90, also known as Thy1 (THYmocyte differentiation antigen 1) is a GPI-linked glycoprotein expressed byendothelial cells, hematopoietic stem cells, and in developing nervoustissue. Unlike human CD90, mouse CD90 is also highly expressed on Tcells and is involved in T cell activation. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse CD90/Thy1 by Western Blot. Western blot shows lysates of mouse thymus tissue, mouse brain tissue, EL‑4 mouse lymphoblast cell line, and CTLL‑2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7335) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD90/Thy1 at approximately 25-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
