| 货号 | AF7320-SP |
| 别名 | CT41.1; CT41.1Cancer/testis antigen 41.1; FLJ21082; tudor domain containing 1; tudor domain containing protein 1; tudor domain-containing protein 1 | 全称 | Tudor Domain Containing 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse TDRD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant human TDRD1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 56165 (Human); 83561 (Mouse); 292129 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse TDRD1 Asn831-Lys960 Accession # Q99MV1 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | TDRD1 (Tudor domain containing protein 1; also MTR-1) is a 130-145 kDa member of the Tudor family of proteins. It is expressed in spermatocytes and spermatids, and forms part of an RNA-silencing complex that regulates gene expression. TDRD1, through its Tudor and MYND domains, binds methylated PIWI (P-element-induced wimpy testis) protein and PIWI-interacting RNA loading factor, respectively. This serves to promote the recruitment of germline-specific small RNAs into a PIWI ribonucleoprotein complex. Mouse TDRD1 is 1172 aa in length. It contains one MYND-type Zn finger region (aa 163-199), and four methyl-binding Tudor domains (aa 307-1032). Over aa 831-960, mouse TDRD1 shares 96% and 66% aa identity with rat and human TDRD1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Mouse TDRD1 by Western Blot. Western blot shows lysates of mouse testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse TDRD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7320) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TDRD1 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
