| 货号 | AF7287-SP |
| 别名 | 18 kDa antrum mucosa protein; AMP18; AMP-18; AMP18BRICHOS domain containing 1; BRICD1; CA11; CA11MGC70354; FOV; Foveolin; gastrokine 1; gastrokine-1; GKN1; Protein CA11 | 全称 | Take out Column D name |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Gastrokine 1 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Gastrokine 1 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 56287 (Human); 66283 (Mouse); 297418 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Gastrokine 1 Tyr38-Tyr201 Accession # Q9CR36 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Gastrokine-1 (GKN1; also CA11 and AMP-18) is an 18-20 kDa member of the Gastrokine protein family. It has limited expression, being restricted to mucous secreting pyloric antrum epithelial cells. Gastrokine-1 appears to promote epithelial cell proliferation and migration, and induce the formation of tight junctions between epithelial cells. By contrast, gastrokine-1 induces Fas expression in tumor cells, resulting in apoptosis. Mature mouse gastrokine-1 is 166 amino acids (aa) in length (aa 36-201). Based on the SwissProt sequence, it possesses one BRICHOS domain (aa 71-165) that contains a mitogenic sequence (aa 113-131). There is one potential alternative start site at Met18. Over aa 38-201, mouse gastrokine-1 shares 65% and 92% aa sequence identity with human and rat gastrokine-1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse and Rat Gastrokine 1 by Western Blot. Western blot shows lysates of mouse stomach tissue and rat stomach tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse Gastrokine 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7287) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Gastrokine 1 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Gastrokine 1 in Human Stomach. Gastrokine 1 was detected in immersion fixed paraffin-embedded sections of human stomach using Sheep Anti-Mouse Gastrokine 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7287) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Gastrokine 1 in Mouse Stomach. Gastrokine 1 was detected in perfusion fixed frozen sections of mouse stomach using Sheep Anti-Mouse Gastrokine 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7287) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to islets. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
