| 货号 | AF7265-SP |
| 别名 | Cadherin family member 3; CDHF3; CDHF3HT-CP; desmocollin 3; desmocollin-4; DSC; DSC1; DSC2; DSC3; DSC4; DSC4desmocollin-3; HT-CP |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Desmocollin-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human Desmocollin-3 is observed, and approximately 5% cross-reactivity with recombinant mouse (rm) Desmocollin-1 and rmDesmocollin-2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1825 (Human); 13507 (Mouse); 307563 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Desmocollin-3 Arg136-Leu695 Accession # P55850 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | DSC-3 (Desmocollin [Greek for "glue-that-binds"]-3) is a 95-110 kDa member of the Ca++-dependent cadherin family of adhesion molecules. It is found on the surface of stratified epithelial cells, including keratinized and nonkeratinized epithelia of the urinary bladder, vagina, oral cavity and skin. DSC-3 serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. In particular, in the extracellular space, DSC-3 forms both homotypic and heterotypic interactions with DSG-1 in-trans, and binds to the cytoskeleton intracellularly via plakophilin-3. Mature mouse DSC-3 is a 760 amino acid (aa) type I transmembrane glycoprotein. The mature molecule contains a 560 aa extracellular region with five cadherin domains (aa 136-691), and a 179 aa cytoplasmic domain that possesses three utilized Ser phosphorylation sites. There is one splice variant that shows an eight aa substitution for aa 831-895. Over aa 136-695, mouse DSC-3 shares 79% and 93% aa identity with human and rat DSC-3, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Desmocollin‑3 by Western Blot. Western blot shows lysates of mouse embryo (15 d.p.c.) tissue and mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Desmocollin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7265) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Desmocollin‑3 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Desmocollin‑3 in Mouse Embryo. Desmocollin‑3 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse Desmocollin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7265) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the epidermis of the developing limb bud. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
