Mouse Spi-B Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Spi-B Affinity Purified Polyclonal Ab (25 UG)

货号： AF7204-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF7204-SP
别名	Spi-B transcription factor (Spi-1/PU.1 related); SPI-B; transcription factor Spi-B
全称	Transcription Factor Spi [Spleen FFV Proviral Integration Oncogene] B
反应种属	Mouse
应用	Immunocytochemistry(5-15 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects mouse Spi-B in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human Spi-B is observed.
使用方法	Immunocytochemistry: 5-15 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6689 (Human); 272382 (Mouse); 499146 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. c-Rel is dispensable for the differentiation and functional maturation of M cells in the follicle-associated epithelium Immunobiology, 2016;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC-Fluorescent
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse Spi-B Tyr18-Glu167 (Tyr110Phe) Accession # O35906
生物活性	Mouse
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	Spi-B (Transcription factor Spi-B) is a 33-45 kDa member of the ets family of transcription factors. It is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC). In transitional B cells, Spi-B promotes their differentiation into follicular (naïve) B cells. In hematopoietic stem cells, Spi-B stimulates the generation of IFN-producing plasmacytoid DC at the expense of T, B and NK cell development. Mouse Spi-B is 267 amino acids (aa) in length. It contains a dual transactivation region (aa 1-62), plus a PEST domain (aa 110-170) and an Ets DNA-binding domain (aa 174-257). There are two isoform variants. One shows a nine aa substitution for aa 1-8, while a second possesses an 18 aa insertion after Leu17. Over aa 18-167, mouse Spi-B shares 91% and 74% aa identity with rat and human Spi-B, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Development and Differentiation Dendritic Cell Development ETS Family Transcription Factors Hematopoietic Stem Cell Markers Hematopoietic Stem Cell Transcription Factors and Regulators ICC / IHC Images: Spi-B Plasmacytoid Dendritic Cells

参考图片

Spi-B in Mouse Splenocytes. Spi-B was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Spi-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7204) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of SPi-B in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse Spi-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7204) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) and Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P). Quadrant markers were set based on control antibody staining (Catalog # 5‑001‑A). To facilitate intracellular staining, cells were fixed with paraformadehyde and permeabilized with saponin.