| 货号 | AF7130-SP |
| 别名 | heat shock factor protein 1; heat shock transcription factor 1HSTF1HSF 1; HSTF 1; HSTF1 | 全称 | Heat Shock Transcription Factor 1 |
| 反应种属 | Mouse |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse HSF1 in direct ELISAs. In direct ELISAs, approximately 6% cross-reactivity with recombinant mouse HSF4 is observed and less than 1% cross-reactivity with recombinant human HSF2 is observed. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3297 (Human); 154991 (Mouse); 79245 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse HSF1 Thr124-Tyr247 Accession # P38532 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | HSF1 (Heat Shock Factor 1; also HSTF 1) is a 67-75 kDa member of the HSF family of proteins. It is constitutively expressed and functions as a transcriptional activator under heat shock conditions. Normally, it exists as an inactive, monomeric member of the HSP multichaperone complex. Following stress, it dissociates from the complex, homotrimerizes, undergoes multiple phosphorylations, and translocates to the nucleus. Nuclear HSF1 is SUMOylated on Lys298 and binds to heat shock elements in select genes such as HSP70. Mouse HSF1 is 525 amino acids (aa) in length. It contains a DNA binding domain (aa 15-120), two hydrophobic oligomerization regions (aa 130-203 and 380-405), a regulatory domain (aa 221-310), and a transactivation domain (aa 367-525). There are at least 13 potential Ser/Thr phosphorylation sites. When highly phosphorylated, HSF1 appears to run at about 82-85 kDa in SDS-PAGE; when SUMOylated, it runs anomalously at around 170 kDa in SDS-PAGE. One splice variant exists that shows a deletion of aa 413-434. Over aa 124-247, mouse HSF1 shares 94% and 99% aa identity with human and rat HSF1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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HSF1 in Mouse Testes. HSF1 was detected in perfusion fixed frozen sections of mouse testes using Goat Anti-Mouse HSF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7130) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the nuclei of spermatocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
