| 货号 | AF6936-SP |
| 别名 | Adult folate-binding protein; FBP; folate binding protein; folate receptor 1 (adult); Folate receptor 1; folate receptor alpha; Folate receptor, adult; Folbp1; FOLR; FR-alpha; KB cells FBP; MOv18; Ovarian tumor-associated antigen MOv18 | 全称 | Folate Receptor 1 |
| 反应种属 | Mouse |
| 应用 | Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse FOLR1 in direct ELISAs. In direct ELISAs, approximately 30% cross-reactivity with recombinant human FOLR1 is observed. |
| 使用方法 | Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2348 (Human); 14275 (Mouse); 171049 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse FOLR1 Arg23-Ser232 Accession # P35846 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Folate receptor 1 (FOLR1; also folate receptor alpha and folate binding protein 1/FOLBP1) is a 40-50 kDa member of the folate receptor family. FOLR1 is expressed in reticulocytes, retinal Muller cells and placenta, plus renal, mammary and thymic epithelium, where it mediates the transport of 5-methyltetrahydrofolate into the cell. The mouse FOLR1 preproprecursor is 255 amino acid (aa) in length. It contains a GPI-linked glycoprotein that is 208 aa in size (aa 25-232), and which possesses three potential N-linked glycosylation sites. There is one potential isoform variant that shows a 105 aa substitution for aa 110-255. Over aa 23-232, mouse FOLR1 shares 83% and 93% aa sequence identity with human and rat FOLR1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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FOLR1 in HeLa Human Cell Line. FOLR1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Mouse FOLR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6936) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
