Mouse RANK/TNFRSF11A Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Mouse RANK/TNFRSF11A Affinity Purified Polyclonal Ab (25 UG)

货号： AF692-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF692-SP
别名	CD265 antigen; CD265; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFR; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TNFRSF11A; TRANCE R; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator
全称	Receptor Activator of NF-kappa-B
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Agonist Activity(0.1-0.4 µg/mL)
目标/特异性	Detects mouse RANK/TNFRSF11A in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human RANK is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Agonist Activity: 0.1-0.4 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8792 (Human); 21934 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. OPG/RANKL/RANK axis is a critical inflammatory signaling system in ischemic brain in mice. Authors: Shimamura M, Nakagami H, Osako M, Kurinami H, Koriyama H, Zhengda P, Tomioka H, Tenma A, Wakayama K, Morishita R Proc Natl Acad Sci U S A, 2014;111(22):8191-6. Species: Mouse Sample Type: Whole Tissue Application: IHC Fluorescence MHC class II transactivator is an in vivo regulator of osteoclast differentiation and bone homeostasis co-opted from adaptive immunity. Authors: Benasciutti E, Mariani E, Oliva L, Scolari M, Perilli E, Barras E, Milan E, Orfanelli U, Fazzalari N, Campana L, Capobianco A, Otten L, Particelli F, Acha-Orbea H, Baruffaldi F, Faccio R, Sitia R, Reith W, Cenci S J Bone Miner Res, 2014;29(2):290-303. Species: Mouse Sample Type: Whole Cells Application: Flow The RANKL signaling axis is sufficient to elicit ductal side-branching and alveologenesis in the mammary gland of the virgin mouse. Authors: Fernandez-Valdivia R, Mukherjee A, Ying Y, Li J, Paquet M, DeMayo FJ, Lydon JP Dev. Biol., 2009;328(1):127-39. Species: Mouse Sample Type: Cell Lysates Application: WB The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis. Authors: Kovacic N, Lukic IK, Grcevic D, Katavic V, Croucher P, Marusic A J. Immunol., 2007;178(6):3379-89. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse RANK/TNFRSF11A Gln30-Pro213 Accession # O35305
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR] and TNFRSF11A) is a member of the tumor necrosis factor receptor family. The full length mouse RANK cDNA encodes a type I transmembrane protein of 625 amino acids (aa) with a predicted 184 aa extracellular domain and a 391 aa cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% aa identity in their extracellular domains. RANK is widely expressed with the highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Anderson, D.M.et al. (1997) Nature 390:175. Nakagawa, N.et al. (1998) Biochem. Biophys. Res. Commun. 245:382.

参考图片

Mouse RANK/TNFRSF11A Antibody Induces Osteoclast Differentiation in Mouse Splenocytes. Mouse RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody induces osteoclast differentiation

on mouse splenocytes in the presence of 20 ng/mL recombinant mouse M-CSF (Catalog # 416-ML), in a dose-dependent manner, as measured by the tartrate-resistant acid phosphatase (TRAP) solution assay. The ED₅₀ for this effect is typically 0.1-0.4 µg/mL.

RANK/TNFRSF11A in Rat Embryo. RANK/TNFRSF11A was detected in immersion fixed frozen sections of rat embryo (cartillage primordium, 7 days p.c.) using 10 µg/mL Goat Anti-Mouse RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF692) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.