| 货号 | AF6879-SP |
| 别名 | PLEXB3; plexin B3; plexin-B3; PLEXR; PLXN6; PLXNB3 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse and rat Plexin B3 in direct ELISAs and Western blots. In direct ELISAs, approximately 38% cross-reactivity with recombinant human (rh) Plexin B3 is observed, and less than 1% cross-reactivity with rhPlexin B2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5365 (Human); 140571 (Mouse); 363517 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin B3 His25-Gln1235 Accession # Q9QY40 |
| 生物活性 | Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Plexin-B3 (also plexin-6) is a 260-270 kDa member of the plexin-B subfamily, plexin family of proteins. It is found on postnatal neurons and oligodendroglia, and appears to have opposing functions. In a context-dependent manner, plexin-B3 may block cell process extension and induce growth cone collapse by binding to Sema5. Conversely, it may engage in homophilic interactions in trans, promoting growth cone extension and axon elongation. The HGF receptor Met has been reported to complex with plexin-B3 in cis, and this interaction may contribute to its reported opposing effects. Mature mouse plexin-B3 is an 1868 amino acid (aa) type I transmembrane glycoprotein. It contains a 1211 aa extracellular domain (ECD) (aa 25-1892) plus a 636 aa cytoplasmic region. The ECD contains one Sema-domain (aa 25-451), two PSI domains (aa 454-646) and four IPT regions (aa 814-1224). There is one isoform variant that contains an alternative Met start site 10 aa upstream of the standard site. Based on human studies, proteolysis of mouse plexin-B3 likely occurs, generating 160 kDa and 140 kDa ECD fragments. Over aa 25-1235, mouse plexin-B3 shares 95% and 82% aa identity with rat and human plexin-B3, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Plexin B3 by Western Blot. Western blot shows lysates of mouse brain (thalamus/hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat Plexin B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6879) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Plexin B3 at approximately 180-190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Plexin B3 in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro‑2A mouse neuroblastoma cell line was stained with Sheep Anti-Mouse/Rat Plexin B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6879, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). | |
Detection of Plexin B3 in C6 Rat Cell Line by Flow Cytometry. C6 rat glioma cell line was stained with Sheep Anti-Mouse/Rat Plexin B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6879, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). | |
Plexin B3 in Mouse Brain. Plexin B3 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse/Rat Plexin B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6879) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to oligodendrocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
