| 货号 | AF6816-SP |
| 别名 | bA325O24.3; bA325O24.4; C6orf190; C6orf207; FLJ40584; Gasp; GRB2-associated protein; MGC163388; protein THEMIS; signaling phosphoprotein specific for T cells; SPOT; thymocyte selection associated; thymocyte selection pathway associated; thymocyte-expressed molecule involved in selection; TSEPA | 全称 | Thymocyte-expressed Molecule Involved in Selection |
| 反应种属 | Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse Themis in direct ELISAs and mouse and rat Themis in Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Themis is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 387357 (Human); 210757 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Themis Lys122-Lys237 Accession # Q8BGW0 |
| 生物活性 | Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Themis (THymus-ExpressedMoleculeInvolved in Selection; also C6orf190, Gasp and Thylex) is a 72-74 kDa member of the Themis family of proteins. It has restricted expression, being found in double negative #2-4 thymocytes, double positive thymocytes, and CD4+ plus CD8+ thymocytes and T cells. The molecule is known to constitute part of the TCR signalosome, along with LAT, Grb2 and PLC gamma 1, and is required for ERK and AP-1 activation. Themis appears to contribute to the process of positive selection. In particular, it is needed to prevent cell death during the selection process. Mouse Themis is 636 amino acids (aa) in length. It contains two globular CABIT (Cys-containing, all-beta in Themis) domains (aa 1-260 and 261-520) plus an NLS (aa 555-563). There are four isoform variants. One shows a 25 aa substitution for aa 238-636, a second possesses an alternative start site at Met90 coupled to a 13 aa substitution for aa 589-636, while third and fourth variants contain 7 aa and 17 aa substitutions for aa 589-636. Over aa 122-237, mouse Themis shares 96% and 87% aa identity with rat and human Themis, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse and Rat Themis by Western Blot. Western blot shows lysates of mouse thymus tissue and Nb2‑11 rat lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat Themis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6816) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Themis at approximately 73 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
