| 货号 | AF6814-SP |
| 别名 | B29; B29B-cell-specific glycoprotein B29; B-cell antigen receptor complex-associated protein beta chain; CD79B antigen (immunoglobulin-associated beta); CD79b antigen; CD79b molecule, immunoglobulin-associated beta; CD79B; IGB; IGBAGM6; Ig-beta; Immunoglobulin-associated B29 protein |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse CD79B in direct ELISAs and Western blots. In direct ELISAs, approximately 55% of cross-reactivity with human CD79B is observed. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 974 (Human); 15985 (Mouse); 171055 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse CD79B Met29-Asp158 Accession # P15530 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | CD79B (also known as B29, Ig beta and B cell antigen receptor complex-associated protein beta -chain) is a 37-39 kDa member of the Ig-Superfamily. It is expressed on B cells, and forms a covalent heterodimer with CD79a. This complex interacts noncovalently with membrane Ig, forming the B cell antigen receptor. Within this complex, membrane Ig detects antigen while CD79a:b initiates signaling. CD79B is also required for formation of pre-B cells during B cell development. Mature mouse CD79B is a 203 amino acid (aa) type I transmembrane glycoprotein (aa 26-228). It contains an extracellular region with one V-type Ig-like domain (aa 41-132) and an ITAM‑containing cytoplasmic domain (aa 181-228). CD79B may migrate as two bands in SDS-PAGE. One defines the standard 37 kDa form, while a second represents one of two possible isoforms, the first of which is an underglycosylated full-length CD79B, and the second of which is an alternative splice form that likely lacks the C‑terminal 32 amino acids. Mouse CD79a and CD79B share only 24% aa identity. Over aa 29-158, mouse CD79B shares 54% and 78% aa identity with human and rat CD79B, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse CD79B by Western Blot. Western blot shows lysates of mouse lymph node tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse CD79B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6814) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD79B at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of CD79B in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse CD79B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6814) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) and Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A). | |
CD79B in Mouse Splenocytes. CD79B was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse CD79B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6814) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
