| 货号 | AF6760-SP |
| 别名 | NID2; NID-2; nidogen 2 (osteonidogen); nidogen-2; Osteonidogen |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.2 µg/mL) |
| 目标/特异性 | Detects mouse Nidogen-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human Nidogen-2 is observed. |
| 使用方法 | Western Blot: 0.2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 22795 (Human); 18074 (Mouse); 302248 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Nidogen-2 Leu31-Lys1403 Accession # NP_032721 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Nidogen-2 (also named entactin-2) is a 160-200 kDa, secreted, monomeric basement membrane glycoprotein (1,2). Nidogens 1 and 2 are expressed in nearly all basement membranes (1-4) where they interact with laminins, collagen type IV and proteoglycan family members to form structural scaffolds (5, 6).Mice deleted for both nidogen genes die at birth due to delayed basement membrane maturation of the lungs and heart (1). However, deletion of one nidogen gives a mild phenotype, indicating that their functions overlap (1, 7). In adult, muscle Nidogen-2 expression is concentrated in the basal lamina of synapses and is required for their maturation and maintenance (8). Both nidogens bind laminin, perlecan and collagens I and IV, but only Nidogen-1 binds fibulins (1, 4). The two nidogens share approximately 40% amino acid (aa) identity in mouse and are structurally similar (1, 2, 5). Cleavage of a 30 aa signal sequence from mouse Nidogen-2 produces a 1373 aa mature protein containing three globular domains (G1-3) separated by a link region and an extended rod-shaped segment. The G1 domain is reported to bind type IV collagen, the G2 nidogen ( beta -barrel) domain interacts with perlecan, and the C-terminal G3 beta -propeller structure is associated with laminin binding. The mucin-like link region is longer in Nidogen-2 than Nidogen-1, and contains both N- and O-glycosylation (3, 8). It contains one EGF-like motif, and an RGD motif that binds alpha 3beta1 integrins. The rod-shaped segment contains four additional EGF-like motifs, two of which bind calcium, and two thyroglobulin type 1 domains that serve as a binding site for alpha vbeta3 integrins. If species‑specific insertions and deletions are disregarded, mature mouse Nidogen-2 shares 80%, 92%, 74%, 73%, 71% and 68% aa identity with human, rat, bovine, porcine, canine and equine Nidogen-2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Nidogen‑2 by Western Blot. Western blot shows lysates of mouse heart tissue and mouse placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse Nidogen‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6760) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Nidogen‑2 at approximately 160-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
