| 货号 | AF6736-SP |
| 别名 | Mel91; nel (chicken)-like 2; NEL-like 2 (chicken); NEL-like protein 2; Nel-related protein 2; NRP2; NRP2neural epidermal growth factor-like 2; protein kinase C-binding protein NELL2 | 全称 | NEL-like 2 |
| 反应种属 | Mouse |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse NELL2 in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) NELL2 and less than 3% cross-reactivity with rhNELL1 is observed. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4753 (Human); 54003 (Mouse); 81734 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse NELL2 Leu25-Leu819 Accession # NP_058023 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | NELL2 (neural EGF-like like protein 2) is a secreted, 130-140 kDa glycoprotein member of the EGF-like domain containing family, Laminin G/N-TSP1/Pentraxin gene superfamily of molecules. It is expressed in both fetal and postnatal neurons, being found in pyramidal and hypothalamic glutamatergic neurons, and in GABAergic cerebellar Purkinje cells. It has multiple functions, including the induction of GnRH release and the promotion of neuronal neurite extension and synapse formation. The mouse NELL2 precursor is 819 amino acids (aa) in length. It contains a 27 aa signal sequence plus a 792 aa mature region. The mature region possesses an N‑terminal TSP domain (aa 33-261), two VWFC domains (aa 275-399), six consecutive EGF-like domains (aa 400-640), and three C-terminal VWFC domains (aa 641‑816). Secreted NELL2 forms 400 kDa noncovalent homotrimers. There are four potential alternate splice forms. One utilizes an alternate start site at Met4, while a second shows a 16 aa substitution for aa 400-436. Based on rat, two other isoforms are likely to exist. A third isoform (cNELL2) should generate a 90 kDa phosphorylated cytosolic molecule that possesses a deletion of aa 22-64, while a fourth, 30 kDa isoform (NELL2-Tsp) should arise due to a premature truncation after Ala261. This last isoform is soluble and predicted to trimerize with full-length NELL2, yielding lower MW complexes. Over aa 24-819, mouse NELL2 shares 97% and 94% aa identity with rat and human NELL2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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NELL2 in Mouse Spinal Cord. NELL2 was detected in perfusion fixed frozen sections of mouse spinal cord using Sheep Anti-Mouse NELL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6736) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to motoneurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
